Filter: AllWBIPIHC

WB analysis using 12436-1-AP (same clone as 12436-1-PBS)
BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12436-1-AP (SHC3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12436-1-PBS in a different storage buffer formulation.
IP experiment of BxPC-3 using 12436-1-AP (same clone as 12436-1-PBS)
IP result of anti-SHC3 (IP:12436-1-AP, 4ug; Detection:12436-1-AP 1:300) with BxPC-3 cells lysate 2800ug. This data was developed using the same antibody clone with 12436-1-PBS in a different storage buffer formulation.
IHC staining of mouse pancreas using 12436-1-AP (same clone as 12436-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 12436-1-AP (SHC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12436-1-PBS in a different storage buffer formulation.
IHC staining of mouse pancreas using 12436-1-AP (same clone as 12436-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 12436-1-AP (SHC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12436-1-PBS in a different storage buffer formulation.

SHC3 Polyclonal antibody, PBS Only

Name: SHC3 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 12436-1-PBS

SHC3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 12436-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

Freight/Packing: -

Quantity

- +

12436-1-PBS targets SHC3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3115 Product name: Recombinant human SHC3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 244-594 aa of BC026314 Sequence: IIANHHMRSISFASGGDPDTTDYVAYVTKDPVNRRACHILECCDGLAQDVIGSIGQAFELRFKQYLQCPTKIPALHDRMQSLDEPWTEEEGDGSDHPYYNSIPSKMPPPGGFLDTRLKPRPHAPDTAQFAGKEQTYYQGRHLGDTFGEDWQQTPLRQGSSDIYSTPEGKLHVAPTGEAPTYVNTQQIPPQAWPAAVSSAESSPRKDLFDMKPFEDALKNQPLGPVLSKAASVECISPVSPRAPDAKMLEELQAETWYQGEMSRKEAEGLLEKDGDFLVRKSTTNPGSFVLTGMHNGQAKHLLLVDPEGTIQTKDRVFDSISHLINHHLESSLPIVSAGSELCLQQPVERKQ Predict reactive species
Full Name	SHC (Src homology 2 domain containing) transforming protein 3
Calculated Molecular Weight	594 aa, 64 kDa
Observed Molecular Weight	50-52 kDa
GenBank Accession Number	BC026314
Gene Symbol	SHC3
Gene ID (NCBI)	53358
RRID	AB_2301798
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q92529
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.