SHC4 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis of fetal human brain using 85181-4-RR (same clone as 85181-4-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 85181-4-RR (SHC4 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85181-4-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 85181-4-RR (same clone as 85181-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SHC4 antibody (85181-4-RR, Clone: 242805E3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85181-4-PBS in a different storage buffer formulation.

• 

  IF Staining of SH-SY5Y using 85181-4-RR (same clone as 85181-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using SHC4 antibody (85181-4-RR, Clone: 242805E3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85181-4-PBS in a different storage buffer formulation.

SHC4 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 85181-4-PBS

Clone No.242805E3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

242805E3, hShcD, Rai like protein, Rai-like protein, RaLP

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Product Information

85181-4-PBS targets SHC4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3336

  Product name: Recombinant human SHC4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 324-630 aa of BC033907

  Sequence: NGMAQDVISTIGQAFELRFKQYLKNPSLNTSCESEEVHIDSHAEEREDHEYYNEIPGKQPPVGGVSDMRIKVQATEQMAYCPIQCEKLCYLPGNSKCSSVYENCLEQSRAIGNVHPRGVQSQRGTSLLKHTCRVDLFDDPCYINTQALQSTPGSAGNQRSAQPLGSPWHCGKAPETVQPGATAQPASSHSLPHIKQQLWSEECYHGKLSRKAAESLLVKDGDFLVRESATSPGQYVLSGLQGGQAKHLLLVDPEGKVRTKDHVFDNVGHLIRYHMDNSLPIISSGSEVSLKQPVRKDNNPALLHSNK

• Full Name: SHC (Src homology 2 domain containing) family, member 4
• Calculated Molecular Weight: 630 aa, 69 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC033907
• Gene Symbol: SHC4
• Gene ID (NCBI): 399694
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q6S5L8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.