SHC4 Recombinant monoclonal antibody

SHC4 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85181-4-RR

Clone No.242805E3

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

242805E3, hShcD, Rai like protein, Rai-like protein, RaLP

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Filter: AllWBIF/ICC

WB analysis of fetal human brain using 85181-4-RR
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 85181-4-RR (SHC4 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis using 85181-4-RR
Various lysates were subjected to SDS PAGE followed by western blot with 85181-4-RR (SHC4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IF Staining of HepG2 using 85181-4-RR
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SHC4 antibody (85181-4-RR, Clone: 242805E3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of SH-SY5Y using 85181-4-RR
Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using SHC4 antibody (85181-4-RR, Clone: 242805E3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	fetal human brain tissue, A375 cells, HepG2 Cells
Positive IF/ICC detected in	HepG2 cells, SH-SY5Y cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85181-4-RR targets SHC4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag3336 Product name: Recombinant human SHC4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 324-630 aa of BC033907 Sequence: NGMAQDVISTIGQAFELRFKQYLKNPSLNTSCESEEVHIDSHAEEREDHEYYNEIPGKQPPVGGVSDMRIKVQATEQMAYCPIQCEKLCYLPGNSKCSSVYENCLEQSRAIGNVHPRGVQSQRGTSLLKHTCRVDLFDDPCYINTQALQSTPGSAGNQRSAQPLGSPWHCGKAPETVQPGATAQPASSHSLPHIKQQLWSEECYHGKLSRKAAESLLVKDGDFLVRESATSPGQYVLSGLQGGQAKHLLLVDPEGKVRTKDHVFDNVGHLIRYHMDNSLPIISSGSEVSLKQPVRKDNNPALLHSNK Predict reactive species
Full Name	SHC (Src homology 2 domain containing) family, member 4
Calculated Molecular Weight	630 aa, 69 kDa
Observed Molecular Weight	75 kDa
GenBank Accession Number	BC033907
Gene Symbol	SHC4
Gene ID (NCBI)	399694
RRID	AB_3744080
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q6S5L8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SHC4 is a member of the SHC family and is characterized by a phosphotyrosine binding (PTB) domain and Src homology 2 (SH2) domain, which both bind to phosphorylated tyrosine residues on cell surface receptors and other signaling proteins. SHC family proteins contain the proline- and glycine-rich domains termed collagen homology 1 (CH1) and CH2 domains, which act as docking sites for certain proteins. Abnormal SHC4 expression promotes cell migration and invasion in melanoma, glioma, and hepatocellular carcinoma (PMID: 38052369).