SHMT1 Polyclonal antibody

SHMT1 Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 30192-1-AP

Synonyms

CSHMT, Serine methylase, SHMT, SHMT1

---

Validation Data Gallery

• 

  WB analysis using 30192-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30192-1-AP (SHMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 30192-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 30192-1-AP (SHMT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 30192-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SHMT1 antibody (30192-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 30192-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SHMT1 (30192-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, MCF-7 cells, MOLT-4 cells, T-47D cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30192-1-AP targets SHMT1 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SHMT1 fusion protein Ag31269
• Full Name: serine hydroxymethyltransferase 1 (soluble)
• Calculated Molecular Weight: 483 aa, 53 kDa
• Observed Molecular Weight: 45-53 kDa
• GenBank Accession Number: BC038598
• Gene Symbol: SHMT1
• Gene ID (NCBI): 6470
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SHMT1 is the cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. Alternative splicing of this gene results in 2 transcript variants encoding 2 different isoforms. This antibody can detect both 49 kDa and 53 kDa isoforms in western blotting.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Adrian (Verified Customer) (03-22-2024)

Very good customer help, quick delivery and good quality antibodies

• Applications: Western Blot