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SHMT1 Recombinant monoclonal antibody, PBS Only (85758-5-PBS)

Filter: AllWBIF/ICCFC (Intra)

WB analysis using 85758-5-RR (same clone as 85758-5-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 85758-5-RR (SHMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85758-5-PBS in a different storage buffer formulation.
WB analysis using 85758-5-RR (same clone as 85758-5-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 85758-5-RR (SHMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85758-5-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 85758-5-RR (same clone as 85758-5-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SHMT1 antibody (85758-5-RR, Clone: 243008E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85758-5-PBS in a different storage buffer formulation.
FC experiment of U2OS using 85758-5-RR (same clone as 85758-5-PBS)
1x10^6 U2OS cells were intracellularly stained with 0.25 ug SHMT1 Recombinant monoclonal antibody (85758-5-RR, Clone:243008E2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 85758-5-PBS in a different storage buffer formulation.
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1x10^6 U2OS cells were intracellularly stained with 0.25 ug SHMT1 Recombinant monoclonal antibody (85758-5-RR, Clone:243008E2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 85758-5-PBS in a different storage buffer formulation.

SHMT1 Recombinant monoclonal antibody, PBS Only

Name: SHMT1 Recombinant monoclonal antibody, PBS Only
Brand: Proteintech
SKU: 85758-5-PBS

SHMT1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 85758-5-PBS

Clone No.243008E2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

CSHMT, EC:2.1.2.1

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

85758-5-PBS targets SHMT1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag31269 Product name: Recombinant human SHMT1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 320-483 aa of BC038598 Sequence: MTLEFKVYQHQVVANCRALSEALTELGYKIVTGGSDNHLILVDLRSKGTDGGRAEKVLEACSIACNKNTCPGDRSALRPSGLRLGTPALTSRGLLEKDFQKVAHFIHRGIELTLQIQSDTGVRATLKEFKERLAGDKYQAAVQALREEVESFASLFPLPGLPDF Predict reactive species
Full Name	serine hydroxymethyltransferase 1 (soluble)
Calculated Molecular Weight	483 aa, 53 kDa
Observed Molecular Weight	45-53 kDa
GenBank Accession Number	BC038598
Gene Symbol	SHMT1
Gene ID (NCBI)	6470
RRID	AB_3744353
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P34896
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

SHMT1 (serine hydroxymethyltransferase 1) is a cytosolic enzyme that plays a central role in one-carbon metabolism, catalyzing the reversible conversion of serine and tetrahydrofolate (THF) to glycine and 5,10-methylenetetrahydrofolate (5,10-methylene-THF). This reaction provides the primary source of one-carbon units for a variety of essential biosynthetic processes, including the synthesis of purines, thymidylate, and methionine, as well as for methylation reactions and redox balance maintenance. SHMT1 is highly expressed in proliferating tissues and cancer cells, where its activity supports increased nucleotide synthesis and cell growth. Additionally, SHMT1 is regulated at both transcriptional and post-translational levels, and its dysregulation has been linked to metabolic disorders, developmental defects, and cancer progression. The enzyme's critical role in compartmentalized one-carbon metabolism makes it an important target for therapeutic intervention, particularly in oncology.