CoraLite® Plus 488-conjugated SHMT1 Monoclonal antibody

SHMT1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rabbit

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D5E6

Cat no : CL488-67963

Synonyms

CSHMT, Serine methylase, SHMT, SHMT1

---

Validation Data Gallery

• 

  IF Staining of A549 using CL488-67963

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 SHMT1 antibody (CL488-67963, Clone: 2D5E6 ) at dilution of 1:200.

• 

  IF Staining of HepG2 using CL488-67963

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 SHMT1 antibody (CL488-67963, Clone: 2D5E6 ) at dilution of 1:200.

---

Tested Applications

• Positive IF detected in: A549 cells, HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67963 targets SHMT1 in IF applications and shows reactivity with Human, pig, rabbit samples.

• Tested Reactivity: Human, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SHMT1 fusion protein Ag5833
• Full Name: serine hydroxymethyltransferase 1 (soluble)
• Calculated Molecular Weight: 483 aa, 53 kDa
• Observed Molecular Weight: 45-53 kDa
• GenBank Accession Number: BC038598
• Gene Symbol: SHMT1
• Gene ID (NCBI): 6470
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.