SHMT2 Monoclonal antibody

SHMT2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3A2H9

Cat no : 67980-1-Ig

Synonyms

GLYA, Serine methylase, SHMT, SHMT2

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Validation Data Gallery

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  WB analysis using 67980-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67980-1-Ig (SHMT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HSC-T6 using 67980-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67980-1-Ig (SHMT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 67980-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67980-1-Ig (SHMT2 antibody) at dilution of 1:1300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67980-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67980-1-Ig (SHMT2 antibody) at dilution of 1:1300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: NCCIT cells, HSC-T6 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
• Positive IHC detected in: human colon cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:650-1:2600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67980-1-Ig targets SHMT2 in WB, IHC, ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SHMT2 fusion protein Ag1584
• Full Name: serine hydroxymethyltransferase 2 (mitochondrial)
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 52-55 kDa
• GenBank Accession Number: BC011911
• Gene Symbol: SHMT2
• Gene ID (NCBI): 6472
• RRID: AB_2918729
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.