Tested Applications
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-67980 targets SHMT2 in IF/ICC, FC (Intra) applications and shows reactivity with human, rat samples.
Tested Reactivity | human, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | SHMT2 fusion protein Ag1584 Predict reactive species |
Full Name | serine hydroxymethyltransferase 2 (mitochondrial) |
Calculated Molecular Weight | 55 kDa |
Observed Molecular Weight | 52-55 kDa |
GenBank Accession Number | BC011911 |
Gene Symbol | SHMT2 |
Gene ID (NCBI) | 6472 |
RRID | AB_2923847 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P34897 |
Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
IF protocol for CL Plus 488 SHMT2 antibody CL488-67980 | Download protocol |
FC protocol for CL Plus 488 SHMT2 antibody CL488-67980 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |