SHQ1 Polyclonal antibody

SHQ1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 27020-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Protein SHQ1 homolog, SHQ1, SHQ1 homolog (S. cerevisiae)

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inK-562 cells, A549 cells, HEK-293T cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman colon cancer tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27020-1-AP targets SHQ1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHQ1 fusion protein Ag25559 Predict reactive species
Full Name SHQ1 homolog (S. cerevisiae)
Calculated Molecular Weight 593 aa, 67 kDa
Observed Molecular Weight 70 kDa~80 kDa
GenBank Accession NumberBC017274
Gene Symbol SHQ1
Gene ID (NCBI) 55164
RRIDAB_2880725
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6PI26
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SHQ1 antibody 27020-1-APDownload protocol
IHC protocol for SHQ1 antibody 27020-1-APDownload protocol
IF protocol for SHQ1 antibody 27020-1-APDownload protocol
IP protocol for SHQ1 antibody 27020-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Nucleic Acids Res

Proteomic analyses reveal new features of the box H/ACA RNP biogenesis

Authors - Florence Schlotter
humanWB,IP,CoIP

Hum Mol Genet

The C-terminal extension of dyskerin is a dyskeratosis congenita mutational hotspot that modulates interaction with telomerase RNA and subcellular localization

Authors - Jian Qin
human,mouseWB

Exp Neurol

Pathogenic SHQ1 variants result in disruptions to neuronal development and the dopaminergic pathway

Authors - Chien-Hui Chang
  • KD Validated
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