Siglec-12 Polyclonal antibody

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  WB analysis using 13964-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13964-1-AP (SIGLEC12 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Siglec-12 Polyclonal Antibody for WB, ELISA

Cat No. 13964-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, ELISA

Synonyms

S2V, Siglec 12, Siglec L1, Siglec XII, SIGLEC12, Siglec-12, SIGLECL1, SLG

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Tested Applications

• Positive WB detected in: mouse spleen tissue, rat spleen tissue, pig spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13964-1-AP targets Siglec-12 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4939

  Product name: Recombinant human SIGLEC12 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 22-350 aa of BC035809

  Sequence: KDYLLTMQKSVTVQEGLCVSVLCSFSYPQNGWTASDPVHGYWFRAGDHVSRNIPVATNNPARAVQEETRDRFHLLGDPQNKDCTLSIRDTRESDAGTYVFCVERGNMKWNYKYDQLSVNVTASQDLLSRYRLEVPESVTVQEGLCVSVPCSVLYPHYNWTASSPVYGSWFKEGADIPWDIPVATNTPSGKVQEDTHGRFLLLGDPQTNNCSLSIRDARKGDSGKYYFQVERGSRKWNYIYDKLSVHVTALTHMPTFSIPGTLESGHPRNLTCSVPWACEQGTPPTITWMGASVSSLDPTITRSSMLSLIPQPQDHGTSLTCQVTLPGAG

• Full Name: sialic acid binding Ig-like lectin 12
• Calculated Molecular Weight: 595 aa, 65 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC035809
• Gene Symbol: SIGLEC12
• Gene ID (NCBI): 89858
• RRID: AB_3669172
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96PQ1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily that contain sialoside-binding N-terminal domains (PMID: 22749793). Sialic acid binding Ig-like lectin 12 (Siglec-12, also known as S2V, SLG, SIGLECL1, and Siglec-XII) is a member of the SIGLEC3-like subfamily of SIGLECs, which is highly expressed in the spleen, such as CD8+ cells, macrophages, neutrophils, and dendritic cells. It mediates sialic-acid-dependent binding to cells and could be an identification of therapeutic targets and prognostic biomarkers of sarcomas (PMID: 38182638).