SIGLEC14 Recombinant monoclonal antibody

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• SIGLEC14 Recombinant monoclonal antibody (86628-3-RR)

• 

  WB analysis using 86628-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86628-3-RR (Siglec-14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of U-937 using 86628-3-RR

  Immunofluorescent analysis of (4% PFA) fixed U-937 cells using SIGLEC14 antibody (86628-3-RR, Clone: 250756E4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

SIGLEC14 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86628-3-RR

Clone No.250756E4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Siglec-14, Sialic acid-binding Ig-like lectin 14, Siglec 14, UNQ294/PRO333

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Tested Applications

• Positive WB detected in: HL-60 cells, THP-1 cells, U-937 cells
• Positive IF/ICC detected in: U-937 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86628-3-RR targets SIGLEC14 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg4178

  Product name: Recombinant Human SIGLEC14 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 17-358 aa of NM_001098612.1

  Sequence: EKPVYELQVQKSVTVQEGLCVLVPCSFSYPWRSWYSSPPLYVYWFRDGEIPYYAEVVATNNPDRRVKPETQGRFRLLGDVQKKNCSLSIGDARMEDTGSYFFRVERGRDVKYSYQQNKLNLEVTALIEKPDIHFLEPLESGRPTRLSCSLPGSCEAGPPLTFSWTGNALSPLDPETTRSSELTLTPRPEDHGTNLTCQVKRQGAQVTTERTVQLNVSYAPQNLAISIFFRNGTGTALRILSNGMSVPIQEGQSLFLACTVDSNPPASLSWFREGKALNPSQTSMSGTLELPNIGAREGGEFTCRVQHPLGSQHLSFILSVQRSSSSCICVTEKQQGSWPLVL

• Full Name: sialic acid binding Ig-like lectin 14
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: NM_001098612.1
• Gene Symbol: SIGLEC14
• Gene ID (NCBI): 100049587
• RRID: AB_3745019
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q08ET2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Siglecs are involved in regulating cell proliferation, differentiation, apoptosis, and implementing cell-cell interactions (PMID: 19076345). They play important roles in regulating immune responses, in hostpathogen interactions, and in tumorigenesis (PMID: 22524423; 25234143). Sialic acid-binding Ig-like lectin 14 (SIGLEC14) is expressed by granulocytes and monocytes, which has an activating role in regulating macrophage immune responses (PMID: 24799499; 37024598).