SIKE1 Polyclonal antibody

SIKE1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 22432-1-AP

Synonyms

RP5 1000E10.4, RP5-1000E10.4, SIKE, SIKE1, Suppressor of IKBKE 1, suppressor of IKK epsilon

---

Validation Data Gallery

• 

  WB analysis of SH-SY5Y using 22432-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 22432-1-AP (SIKE1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 22432-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 22432-1-AP (SIKE1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 22432-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22432-1-AP (SIKE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22432-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22432-1-AP (SIKE1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: SH-SY5Y cells, HEK-293 cells, MCF-7 cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22432-1-AP targets SIKE1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIKE1 fusion protein Ag18196
• Full Name: suppressor of IKK epsilon
• Calculated Molecular Weight: 207 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC005934
• Gene Symbol: RP5-1000E10.4
• Gene ID (NCBI): 80143
• RRID: AB_10914502
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.