SIM2 Polyclonal antibody

SIM2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21069-1-AP

Synonyms

bHLHe15, SIM, SIM2, Single minded homolog 2

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Validation Data Gallery

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  WB analysis using 21069-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21069-1-AP (SIM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 21069-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21069-1-AP (SIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 21069-1-AP

  IP result of anti-SIM2 (IP:21069-1-AP, 4ug; Detection:21069-1-AP 1:800) with mouse skeletal muscle tissue lysate 2200ug.

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Tested Applications

• Positive WB detected in: COLO 320 cells, human skeletal muscle tissue, HeLa cells, HepG2 cells, U-251 cells
• Positive IP detected in: mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• ChIP: See 1 publications below

Product Information

21069-1-AP targets SIM2 in WB, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIM2 fusion protein Ag15204
• Full Name: single-minded homolog 2 (Drosophila)
• Calculated Molecular Weight: 667 aa, 73 kDa
• Observed Molecular Weight: 60-64 kDa
• GenBank Accession Number: BC110444
• Gene Symbol: SIM2
• Gene ID (NCBI): 6493
• RRID: AB_10732844
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIM2, also named as single-minded homolog 2, is a 667 amino acid protein, which contains 1 bHLH (basic helix-loop-helix) domain and one PAS-associated C-terminal domain. SIM2 localizes in the nucleus and has tow isoforms. Isoform SIM2 is a 73 kDa protein and Isoform SIM2S is 63 kDa protein. SIM2 as a transcription factor may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tissues expressed during development.

Publications

Species	Application	Title
human	ChIP	Biochem J; Characterization of functionally deficient SIM2 variants found in patients with neurological phenotypes.; Authors - Emily Lyn Button