SIM2 Polyclonal antibody

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• SIM2 Polyclonal antibody (21069-1-AP)

SIM2 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 21069-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ChIP, ELISA

Synonyms

Class E basic helix-loop-helix protein 15, SIM, Single minded homolog 2, Single-minded homolog 2

• 

  WB analysis using 21069-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21069-1-AP (SIM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human skeletal muscle using 21069-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21069-1-AP (SIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse skeletal muscle using 21069-1-AP

  IP result of anti-SIM2 (IP:21069-1-AP, 4ug; Detection:21069-1-AP 1:800) with mouse skeletal muscle tissue lysate 2200ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 21069-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21069-1-AP (SIM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: COLO 320 cells, human skeletal muscle tissue, HeLa cells, HepG2 cells, U-251 cells
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21069-1-AP targets SIM2 in WB, IHC, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15204

  Product name: Recombinant human SIM2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 342-525 aa of BC110444

  Sequence: LSLEQVSTAKSQDSWRTALSTSQETRKLVKPKNTKMKTKLRTNPYPPQQYSSFQMDKLECGQLGNWRASPPASAAAPPELQPHSESSDLLYTPSYSLPFSYHYGHFPLDSHVFSSKKPMLPAKFGQPQGSPCEVARFFLSTLPASGECQWHYANPLVPSSSSPAKNPPEPPANTARHSLVPSYE

• Full Name: single-minded homolog 2 (Drosophila)
• Calculated Molecular Weight: 667 aa, 73 kDa
• Observed Molecular Weight: 60-64 kDa
• GenBank Accession Number: BC110444
• Gene Symbol: SIM2
• Gene ID (NCBI): 6493
• RRID: AB_10732844
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14190
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIM2, also named as single-minded homolog 2, is a 667 amino acid protein, which contains 1 bHLH (basic helix-loop-helix) domain and one PAS-associated C-terminal domain. SIM2 localizes in the nucleus and has tow isoforms. Isoform SIM2 is a 73 kDa protein and Isoform SIM2S is 63 kDa protein. SIM2 as a transcription factor may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tissues expressed during development.

Publications

What published studies show

The SIM2 Polyclonal antibody (Cat# 21069-1-AP) has 1 total citation. It was cited in Biochemical Journal (2022) in a study characterizing functionally deficient SIM2 variants in patients with neurological phenotypes, using the ChIP application. The research field is neurology, specifically related to CNS development and neurological disorders associated with SIM2 mutations.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	ChIP	Biochem J; Characterization of functionally deficient SIM2 variants found in patients with neurological phenotypes.; Authors - Emily Lyn Button