SIM2 Polyclonal antibody, PBS Only

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• SIM2 Polyclonal antibody, PBS Only (21069-1-PBS)

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  WB analysis using 21069-1-AP (same clone as 21069-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21069-1-AP (SIM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21069-1-PBS in a different storage buffer formulation.

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  WB analysis of human skeletal muscle using 21069-1-AP (same clone as 21069-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21069-1-AP (SIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21069-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse skeletal muscle using 21069-1-AP (same clone as 21069-1-PBS)

  IP result of anti-SIM2 (IP:21069-1-AP, 4ug; Detection:21069-1-AP 1:800) with mouse skeletal muscle tissue lysate 2200ug. This data was developed using the same antibody clone with 21069-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 21069-1-AP (same clone as 21069-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21069-1-AP (SIM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21069-1-PBS in a different storage buffer formulation.

SIM2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 21069-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Class E basic helix-loop-helix protein 15, SIM, Single minded homolog 2, Single-minded homolog 2

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Product Information

21069-1-PBS targets SIM2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15204

  Product name: Recombinant human SIM2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 342-525 aa of BC110444

  Sequence: LSLEQVSTAKSQDSWRTALSTSQETRKLVKPKNTKMKTKLRTNPYPPQQYSSFQMDKLECGQLGNWRASPPASAAAPPELQPHSESSDLLYTPSYSLPFSYHYGHFPLDSHVFSSKKPMLPAKFGQPQGSPCEVARFFLSTLPASGECQWHYANPLVPSSSSPAKNPPEPPANTARHSLVPSYE

• Full Name: single-minded homolog 2 (Drosophila)
• Calculated Molecular Weight: 667 aa, 73 kDa
• Observed Molecular Weight: 60-64 kDa
• GenBank Accession Number: BC110444
• Gene Symbol: SIM2
• Gene ID (NCBI): 6493
• RRID: AB_10732844
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14190
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SIM2, also named as single-minded homolog 2, is a 667 amino acid protein, which contains 1 bHLH (basic helix-loop-helix) domain and one PAS-associated C-terminal domain. SIM2 localizes in the nucleus and has tow isoforms. Isoform SIM2 is a 73 kDa protein and Isoform SIM2S is 63 kDa protein. SIM2 as a transcription factor may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tissues expressed during development.