Tested Applications
Positive WB detected in | U-251 cells, MCF-7 cells, HaCaT cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
85702-4-RR targets SIN1/MAPKAP1 in WB, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | SIN1/MAPKAP1 fusion protein Ag7753 Predict reactive species |
Full Name | mitogen-activated protein kinase associated protein 1 |
Calculated Molecular Weight | 59 kDa |
Observed Molecular Weight | 65 kDa |
GenBank Accession Number | BC002326 |
Gene Symbol | MAPKAP1 |
Gene ID (NCBI) | 79109 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9BPZ7 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Mitogen-activated protein kinase 2-associated protein 1 (MAPKAP1) is also named as MIP1 and SIN1. SIN1/MAPKAP1 is a major partner of mTORC2, acting as a scaffold and responsible for the substrate specificity of the mTOR catalytic subunit (PMID: 37877351). Besides as a component of MTORC2, MAPKAP1 also functions as MEKK2 and Ras-interacting protein and involves in regulating MEKK2 activation and RAS signaling (PMID: 31773361).
Protocols
Product Specific Protocols | |
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WB protocol for SIN1/MAPKAP1 antibody 85702-4-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |