SIN3A Polyclonal antibody

SIN3A Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14638-1-AP

Synonyms

KIAA0700, SIN3A

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Validation Data Gallery

• 

  WB analysis of Jurkat using 14638-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14638-1-AP (SIN3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14638-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14638-1-AP (SIN3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14638-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14638-1-AP (SIN3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14638-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14638-1-AP (SIN3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 14638-1-AP

  IP Result of anti-SIN3A (IP:14638-1-AP, 4ug; Detection:14638-1-AP 1:500) with Jurkat cells lysate 3600ug.

• 

  IF Staining of HepG2 using 14638-1-AP

  Immunofluorescent analysis of HepG2 cells, using SIN3A antibody 14638-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells, HeLa cells, K-562 cells
• Positive IP detected in: Jurkat cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below
• CoIP: See 1 publications below

Product Information

14638-1-AP targets SIN3A in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIN3A fusion protein Ag6219
• Full Name: SIN3 homolog A, transcription regulator (yeast)
• Calculated molecular weight: 145 kDa
• Observed molecular weight: 150 kDa
• GenBank accession number: BC066364
• Gene symbol: SIN3A
• Gene ID (NCBI): 25942
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Theranostics; SLC14A1 prevents oncometabolite accumulation and recruits HDAC1 to transrepress oncometabolite genes in urothelial carcinoma.; Authors - Ti-Chun Chan
human	WB,CoIP	Int J Biol Sci; TET1s deficiency exacerbates oscillatory shear flow-induced atherosclerosis.; Authors - Kai Qu