SIRP Alpha/CD172a Polyclonal antibody

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  WB analysis using 31485-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31485-1-AP (SIRP Alpha/CD172a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 31485-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 31485-1-AP (SIRP Alpha antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of THP-1 using 31485-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using SIRP Alpha/CD172a antibody (31485-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of THP-1 using 31485-1-AP

  1x10^6 THP-1 cells were intracellularly stained with 0.25 ug SIRP Alpha/CD172a Polyclonal antibody (31485-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

SIRP Alpha/CD172a Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 31485-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

SIRP Alpha, SIRPA, BIT, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A

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Tested Applications

• Positive WB detected in: C6 cells, NIH/3T3 cells, U-937 cells
• Positive IF/ICC detected in: THP-1 cells
• Positive FC (Intra) detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31485-1-AP targets SIRP Alpha/CD172a in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Eg0625

  Product name: Recombinant Human SIRP alpha/CD172a protein (His Tag)(HPLC verified)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 31-370 aa of NM_080792

  Sequence: EEELQVIQPDKSVLVAAGETATLRCTATSLIPVGPIQWFRGAGPGRELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRIGNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVSGPAARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNER

• Full Name: signal-regulatory protein alpha
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 65-100 kDa
• GenBank Accession Number: NM_080792
• Gene Symbol: SIRP Alpha
• Gene ID (NCBI): 140885
• RRID: AB_3670002
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P78324
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).