CoraLite® Plus 647 Anti-Human SIRP beta 1/CD172b Rabbit Recombinant Antibody

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  FC experiment of human peripheral blood leukocytes using CL647-98267

  1x10^6 human peripheral blood leukocytes were surface stained with CoraLite® Plus 488 Anti-Human CD14, and 5 ul CoraLite® Plus 647 Anti-Human SIRP beta 1/CD172b Rabbit RecAb (CL647-98267, Clone: 242115C10) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using CL647-98267

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Human SIRP beta 1/CD172b Rabbit RecAb (CL647-98267, Clone: 242115C10) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

SIRP beta 1/CD172b Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL647-98267

Clone No.242115C10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

SIRP BETA 1, SIRPB1, 242115C10, CD172 antigen-like family member B, CD172b

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98267 targets SIRP beta 1/CD172b in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SIRP beta 1/CD172b fusion protein Eg1668
• Full Name: signal-regulatory protein beta 1
• Calculated Molecular Weight: 43kDa
• GenBank Accession Number: NM_006065.5
• Gene Symbol: SIRPB1
• Gene ID (NCBI): 10326
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O00241-1
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

SIRP beta 1, also known as CD172b, is a member of the signal-regulatory-protein (SIRP) family and also belongs to the immunoglobulin superfamily. SIRP family members are cell surface signaling receptors differentially expressed in leukocytes and the central nervous system. SIRP beta 1 interacts with TYROBP/DAP12, a protein-bearing immunoreceptor tyrosine-based activation motif. SIRP beta 1 also participates in the recruitment of tyrosine kinase SYK.