SIRP Alpha/CD172a Recombinant monoclonal antibody, PBS Only

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  IF Staining of THP-1 using 83729-3-RR (same clone as 83729-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using SIRP Alpha/CD172a antibody (83729-3-RR, Clone: 240789E7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83729-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83729-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83729-3-RR against Human SIRP Alpha were performed. The affinity constant is 1.37 nM.

SIRP Alpha/CD172a Uni-rAb^® Recombinant Antibody for IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 83729-3-PBS

Clone No.240789E7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

SIRP Alpha, SIRPA, 240789E7, BIT, Brain Ig-like molecule with tyrosine-based activation motifs

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Product Information

83729-3-PBS targets SIRP Alpha/CD172a in IF/ICC, Cytometric bead array, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0625

  Product name: Recombinant Human SIRP alpha/CD172a protein (His Tag)(HPLC verified)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 31-370 aa of NM_080792

  Sequence: EEELQVIQPDKSVLVAAGETATLRCTATSLIPVGPIQWFRGAGPGRELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRIGNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVSGPAARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNER

• Full Name: signal-regulatory protein alpha
• Calculated Molecular Weight: 55 kDa
• GenBank Accession Number: NM_080792
• Gene Symbol: SIRP Alpha
• Gene ID (NCBI): 140885
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P78324
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).