Filter: AllWBIPIF/ICC

WB analysis of A2780 using 10709-1-AP (same clone as 10709-1-PBS)
A2780 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10709-1-PBS in a different storage buffer formulation.
WB analysis of SKOV-3 using 10709-1-AP (same clone as 10709-1-PBS)
SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10709-1-PBS in a different storage buffer formulation.
WB analysis of SKOV-3 using 10709-1-AP (same clone as 10709-1-PBS)
SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10709-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 10709-1-AP (same clone as 10709-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10709-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 10709-1-AP (same clone as 10709-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10709-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 10709-1-AP (same clone as 10709-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10709-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 10709-1-AP (same clone as 10709-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10709-1-PBS in a different storage buffer formulation.
WB analysis of PC-3 using 10709-1-AP (same clone as 10709-1-PBS)
PC-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10709-1-PBS in a different storage buffer formulation.
WB analysis of PC-3 using 10709-1-AP (same clone as 10709-1-PBS)
PC-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10709-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 10709-1-AP (same clone as 10709-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10709-1-PBS in a different storage buffer formulation.
WB analysis of L02 using 10709-1-AP (same clone as 10709-1-PBS)
L02 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10709-1-PBS in a different storage buffer formulation.
WB analysis of L02 using 10709-1-AP (same clone as 10709-1-PBS)
L02 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10709-1-PBS in a different storage buffer formulation.
WB analysis of L02 using 10709-1-AP (same clone as 10709-1-PBS)
L02 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10709-1-PBS in a different storage buffer formulation.
IP experiment of HEK-293 using 10709-1-AP (same clone as 10709-1-PBS)
IP result of anti-SIX1 (IP:10709-1-AP, 3ug; Detection:10709-1-AP 1:1000) with HEK-293 cells lysate 2560ug. This data was developed using the same antibody clone with 10709-1-PBS in a different storage buffer formulation.
IF Staining of epithelial tumor cells using 10709-1-AP (same clone as 10709-1-PBS)
IF result of anti-SIX1 (1:50) in p53-/- mammary epithelial tumor cells. Blue: hoechst nuclear stain, Red: SIX1 stain the mesenchymal cell which have undergone EMT. This data was developed using the same antibody clone with 10709-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

SIX1 Polyclonal antibody, PBS Only

SIX1 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Cat No. 10709-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

10709-1-PBS targets SIX1 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1044 Product name: Recombinant human SIX1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-284 aa of BC008874 Sequence: MSMLPSFGFTQEQVACVCEVLQQGGNLERLGRFLWSLPACDHLHKNESVLKAKAVVAFHRGNFRELYKILESHQFSPHNHPKLQQLWLKAHYVEAEKLCGRPLGAVGKYRVRRKFPLPRTIWDGEETSYCFKEKSRGVLREWYAHNPYPSPREKRELAEATGLTTTQVSNWFKNRRQRDRAAEAKERENTENNNSSSNKQNQLSPLEGGKPLMSSSEEEFSPPQSPDQNSVLLLQGNMGHARSSNYSLPGLTASQPSHGLQTHQHQLQDSLLGPLTSSLVDLGS Predict reactive species
Full Name	SIX homeobox 1
Calculated Molecular Weight	32 kDa
Observed Molecular Weight	32-35 kDa
GenBank Accession Number	BC008874
Gene Symbol	SIX1
Gene ID (NCBI)	6495
RRID	AB_2189077
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q15475
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

SIX1 belongs to SIX family that contains a divergent DNA-binding homeodomain and an upstream SIX domain, which may participate both in determining DNA-binding specificity and in mediating protein-protein interactions. It may be involved in limb tendon and ligament development, and specifically expressed in skeletal muscle. SIX1 plays a essential role in the development of numerous organs and shows littlt to no expression in most non-neoplastic adult tissue, yet is overexpression in a number of meoplasms where it increases cell proliferation and survival. The calcualted molecular weight of SIX1 is 32kDa, but modified SIX1 is about 35 kDa.