SIX4 Polyclonal antibody, PBS Only

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• SIX4 Polyclonal antibody, PBS Only (21305-1-PBS)

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  WB analysis using 21305-1-AP (same clone as 21305-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21305-1-AP (SIX4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21305-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 21305-1-AP (same clone as 21305-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 21305-1-AP (SIX4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21305-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse tongue using 21305-1-AP (same clone as 21305-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 21305-1-AP (SIX4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21305-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse salivary gland using 21305-1-AP (same clone as 21305-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse salivary gland tissue slide using 21305-1-AP (SIX4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21305-1-PBS in a different storage buffer formulation.

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  IHC staining of rat salivary gland using 21305-1-AP (same clone as 21305-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat salivary gland tissue slide using 21305-1-AP (SIX4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21305-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 21305-1-AP (same clone as 21305-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21305-1-AP (SIX4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21305-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 21305-1-AP (same clone as 21305-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21305-1-AP (SIX4 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21305-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 21305-1-AP (same clone as 21305-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21305-1-AP (SIX4 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21305-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 21305-1-AP (same clone as 21305-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SIX4 antibody (21305-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 21305-1-PBS in a different storage buffer formulation.

SIX4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 21305-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

AREC3, Homeobox protein SIX4, SIX homeobox 4

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Product Information

21305-1-PBS targets SIX4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15918

  Product name: Recombinant human SIX4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 411-760 aa of BC098282

  Sequence: TTTSYSPSVPVSFPGLIPSTEVKREGIQTVASQDGGSVVTFTTPVQINQYGIVQIPNSGANSQFLNGSIGFSPLQLPPVSVAASQGNISVSSSTSDGSTFTSESTTVQQGKVFLSSLAPSAVVYTVPNTGQTIGSVKQEGLERSLVFSQLMPVNQNAQVNANLSSENISGSGLHPLASSLVNVSPTHNFSLSPSTLLNPTELNRDIADSQPMSAPVASKSTVTSVSNTNYATLQNCSLITGQDLLSVPMTQAALGEIVPTAEDQVGHPSPAVHQDFVQEHRLVLQSVANMKENFLSNSESKATSSLMMLDSKSKYVLDGMVDTVCEDLETDKKELAKLQTVQLDEDMQDL

• Full Name: SIX homeobox 4
• Calculated Molecular Weight: 781 aa, 83 kDa
• Observed Molecular Weight: 98 kDa
• GenBank Accession Number: BC098282
• Gene Symbol: SIX4
• Gene ID (NCBI): 51804
• RRID: AB_10860258
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UIU6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The Six (sine oculis) proteins are a family of homeodomain transcription factors that share a conserved DNA-binding domain and are human homologs of the Drosophila sine oculis (so) protein. SIX homeobox 4 (SIX4), also known as AREC3, belongs to the the Six family. Six4 contains one Six domain (which funtions as a homeobox DNA-binding motif) and shares 90% sequence similarity with its mouse counterpart, suggesting that both proteins have similar DNA-binding properties. SIX4 is thought to be involved in neurogenesis, myogenesis, and development of other organs, based on its specific expression in certain neuronal cells of the developing embryo and in adult skeletal muscles.