SIX6 Monoclonal antibody

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• SIX6 Monoclonal antibody (67622-1-Ig)

SIX6 Monoclonal Antibody for WB, ELISA

Cat No. 67622-1-Ig Copied!

Clone No. 1C12E2

Publications (1)

Download datasheet

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, ELISA

Synonyms

1C12E2, Homeobox protein SIX6, OPTX2, Six9

• 

  WB analysis of HUVEC using 67622-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 67622-1-Ig (SIX6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of NCCIT using 67622-1-Ig

  NCCIT cells were subjected to SDS PAGE followed by western blot with 67622-1-Ig (SIX6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HUVEC cells, NCCIT cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67622-1-Ig targets SIX6 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15496

  Product name: Recombinant human SIX6 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 192-246 aa of BC069413

  Sequence: LQQQVLSQGSGRALRAEGDGTPEVLGVATSPAASLSSKAATSAISITSSDSECDI

• Full Name: SIX homeobox 6
• Calculated Molecular Weight: 246 aa, 28 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC069413
• Gene Symbol: SIX6
• Gene ID (NCBI): 4990
• RRID: AB_2882824
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O95475
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIX6, also named as OPTX2 or SIX9, is a 246 amino acid protein, which is expressed in the developing and adult retina. Defects in this gene are a cause of isolated microphthalmia with cataract type 2 (MCOPCT2).

Publications

What published studies show

The SIX6 Monoclonal antibody (1C12E2), catalog number 67622-1-Ig, has 1 total citation. It appears in Scientific Reports (Impact Factor 4.9, 2025). The cited study investigated how SIX1 aggravates spinal cord injury progression in mice by promoting M1 polarization of microglia, placing this citation in the fields of neuroscience and neuroinflammation. The antibody was used in Western Blot (WB) with mouse samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Sci Rep; SIX1 aggravates the progression of spinal cord injury in mice by promoting M1 polarization of microglia; Authors - Zhonghua Xu