SLAMF6 Polyclonal antibody

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• SLAMF6 Polyclonal antibody (33396-1-AP)

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  WB analysis using 33396-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33396-1-AP (SLAMF6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 33396-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 33396-1-AP (SLAMF6 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 33396-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 33396-1-AP (SLAMF6 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 33396-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 33396-1-AP (SLAMF6 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 33396-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 33396-1-AP (SLAMF6 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLAMF6 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 33396-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Synonyms

CD352, Ly108, Lymphocyte antigen 108, SLAM family member 6

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Tested Applications

• Positive WB detected in: mouse spleen tissue, mouse thymus tissue
• Positive IHC detected in: mouse spleen tissue, mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:4000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33396-1-AP targets SLAMF6 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Eg5249

  Product name: Recombinant Mouse SLAMF6/CD352 protein (rFc Tag)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 31-239 aa of NM_030710.2

  Sequence: EVSQSSSDPQLMNGVLGESAVLPLKLPAGKIANIIIWNYEWEASQVTALVINLSNPESPQIMNTDVKKRLNITQSYSLQISNLTMADTGSYTAQITTKDSEVITFKYILRVFERLGNLETTNYTLLLENGTCQIHLACVLKNQSQTVSVEWQATGNISLGGPNVTIFWDPRNSGDQTYVCRAKNAVSNLSVSVSTQSLCKGVLTNPPWN

• Full Name: SLAM family member 6
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_030710.2
• Gene Symbol: Slamf6
• Gene ID (NCBI): 30925
• RRID: AB_3742959
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9ET39-2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Signaling lymphocyte activation molecule 6 (SLAMF6) (Ly108 in mice, NTB-A or SF2000 in humans) is a homophilic receptor belonging to the superfamily immunoglobulin (Ig) domain-containing molecules (PMID:31199820). SLAMF6 is a type I transmembrane protein with two extracellular immunoglobins (Ig)-like domains and three cytoplasmic tyrosine-based signaling motifs, one of which is immunoreceptor tyrosine-based switch motif (PMID:31315913). SLAMF6 is expressed on a wide variety of immune cells including T cells (also TFH), B cells, NK cells (expressed in humans only), double positive thymocytes, eosinophils, and neutrophils (mouse only) (PMID:11862385).