SLC10A3 Recombinant monoclonal antibody

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  WB analysis of pig liver using 84843-1-RR

  pig liver tissue were subjected to SDS PAGE followed by western blot with 84843-1-RR (SLC10A3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 84843-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84843-1-RR against Human SLC10A3 were performed. The affinity constant is 4.48 nM.

SLC10A3 Recombinant Antibody for WB, ELISA

Cat No. 84843-1-RR

Clone No.241478A8

Host / Isotype

Rabbit / IgG

Reactivity

human, pig

Applications

WB, ELISA

Synonyms

P3, DXS253E, 241478A8

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Tested Applications

• Positive WB detected in: pig liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84843-1-RR targets SLC10A3 in WB, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag13915

  Product name: Recombinant human SLC10A3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-185 aa of BC004966

  Sequence: MVLMQDKGSSQQWPGLGGEGGGTGPLSMLRAALLLISLPWGAQGTASTSLSTAGGHTVPPTGGRYLSIGDGSVMEFEFPEDSEGIIVISSQYPGQANRTAPGPMLRVTSLDTEVLTIKNLVDAHEAPPTLIEERRDFCIKVSPAEDTPATLSADLAHFSENPILYLLLPLIFVNKCSFGCKVELE

• Full Name: solute carrier family 10 (sodium/bile acid cotransporter family), member 3
• Calculated Molecular Weight: 477 aa, 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC004966
• Gene Symbol: SLC10A3
• Gene ID (NCBI): 8273
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P09131
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The solute carrier (SLC) family 10 (SLC10) encodes protein for transporters of various agents, bile acids and steroidal hormones, and SLC01A3 belongs to the SLC10 family. SLC10A3 consists of 477 amino acids, with a calculated mass of approximately 50 kDa.