Tested Applications
| Positive WB detected in | HEK-293 cells, 37°C incubated mouse kidney tissue |
| Positive IHC detected in | mouse kidney tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse Embryonic kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18970-1-AP targets SLC12A1/NKCC2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5325 Product name: Recombinant human SLC12A1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-176 aa of BC040138 Sequence: MSLNNSSNVFLDSVPSNTNRFQVSVINENHESSAAADDNTDPPHYEETSFGDEAQKRLRISFRPGNQECYDNFLQSGETAKTDASFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSISGPKVNRPSLLEIHEQLAKNVAVTPSSADRVANGDGIPGDEQAENKEDDQAGVVK Predict reactive species |
| Full Name | solute carrier family 12 (sodium/potassium/chloride transporters), member 1 |
| Calculated Molecular Weight | 121 kDa |
| Observed Molecular Weight | 135 kDa |
| GenBank Accession Number | BC040138 |
| Gene Symbol | SLC12A1/NKCC2 |
| Gene ID (NCBI) | 6557 |
| RRID | AB_2877130 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13621 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SLC12A1/NKCC2 antibody 18970-1-AP | Download protocol |
| IHC protocol for SLC12A1/NKCC2 antibody 18970-1-AP | Download protocol |
| WB protocol for SLC12A1/NKCC2 antibody 18970-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SLC12A1/NKCC2 polyclonal antibody (Cat# 18970-1-AP) has accumulated 41 citations in high-impact journals including Cell Stem Cell, Nature Protocols, Nature Communications, Theranostics, Molecular Therapy, Hypertension, Journal of the American Society of Nephrology, Development, iScience, and Scientific Reports. Research fields covered include nephron development, kidney organoid modeling, proximal tubule physiology, acute kidney injury, hypertension, polycystic kidney disease, and renal cell signaling pathways.
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Plasticity of distal nephron epithelia from human kidney organoids enables the induction of ureteric tip and stalk. | ||
Cell Stem Cell Genetics of cystogenesis in base-edited human organoids reveal therapeutic strategies for polycystic kidney disease | ||
Nat Protoc Generation of proximal tubule-enhanced kidney organoids from human pluripotent stem cells | ||
Nat Commun Enhanced metanephric specification to functional proximal tubule enables toxicity screening and infectious disease modelling in kidney organoids | ||
Theranostics WT1+ glomerular parietal epithelial progenitors promote renal proximal tubule regeneration after severe acute kidney injury |
