SLC12A3/NCCT Polyclonal antibody

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• SLC12A3/NCCT Polyclonal antibody (55442-1-AP)

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  WB analysis using 55442-1-AP

  37°C incubated mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55442-1-AP (SLC12A3/NCCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 55442-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55442-1-AP (SLC12A3/NCCT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 55442-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55442-1-AP (SLC12A3/NCCT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLC12A3/NCCT Polyclonal Antibody for WB, IHC, ELISA

Cat No. 55442-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

SLC12A3, NCCT

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Tested Applications

• Positive WB detected in: 37°C incubated mouse kidney tissue
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

For optimal WB detection with 55442-1-AP, we do not recommend boiling the sample after lysis.

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55442-1-AP targets SLC12A3/NCCT in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: solute carrier family 12 (sodium/chloride transporters), member 3
• Calculated Molecular Weight: 114 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: NM_000339
• Gene Symbol: SLC12A3
• Gene ID (NCBI): 6559
• RRID: AB_3743209
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P55017
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC12A3 also known as NCCT, NCC, is a sodium-chloride cotransporter (NCC), which mainly functions in the distal convoluted tubules of the kidney and is responsible for the reabsorption of sodium ions and chloride ions (PMID: 35591852). This cotransporter is the target of thiazide diuretics used in the treatment of hypertension. Mutations in SLC12A3 cause Gitelman syndrome(PMID: 8528245, 33462018).