SLC12A4/KCC1 Polyclonal antibody, PBS Only

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• SLC12A4/KCC1 Polyclonal antibody, PBS Only (15927-1-PBS)

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  WB analysis of HeLa using 15927-1-AP (same clone as 15927-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15927-1-AP (SLC12A4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15927-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 15927-1-AP (same clone as 15927-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15927-1-AP (SLC12A4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15927-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 15927-1-AP (same clone as 15927-1-PBS)

  IP result of anti-SLC12A4 (IP:15927-1-AP, 4ug; Detection:15927-1-AP 1:500) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 15927-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 15927-1-AP (same clone as 15927-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15927-1-AP (SLC12A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15927-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 15927-1-AP (same clone as 15927-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15927-1-AP (SLC12A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15927-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 15927-1-AP (same clone as 15927-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15927-1-AP (SLC12A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15927-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 15927-1-AP (same clone as 15927-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15927-1-AP (SLC12A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15927-1-PBS in a different storage buffer formulation.

SLC12A4/KCC1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 15927-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

SLC12A4, Electroneutral potassium-chloride cotransporter 1, Erythroid K-Cl cotransporter 1, KCC1

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Product Information

15927-1-PBS targets SLC12A4/KCC1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8762

  Product name: Recombinant human SLC12A4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 891-1085 aa of BC021193

  Sequence: MKKDLAVFLYHLRLEAEVEVVEMHNSDISAYTYERTLMMEQRSQMLRQMRLTKTEREREAQLVKDRHSALRLESLYSDEEDESAVGADKIQMTWTRDKYMTETWDPSHAPDNFRELVHIKPDQSNVRRMHTAVKLNEVIVTRSHDARLVLLNMPGPPRNSEGDENYMEFLEVLTEGLERVLLVRGGGREVITIYS

• Full Name: solute carrier family 12 (potassium/chloride transporters), member 4
• Calculated Molecular Weight: 1085 aa, 121 kDa
• Observed Molecular Weight: 121 kDa
• GenBank Accession Number: BC021193
• Gene Symbol: SLC12A4
• Gene ID (NCBI): 6560
• RRID: AB_2188666
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UP95
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.