SLC13A2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SLC13A2 Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 21722-1-AP

Synonyms

NaCT, NaDC 1, NADC1, SDCT1, SLC13A2

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Validation Data Gallery

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  IP experiment of mouse kidney using 21722-1-AP

  IP result of anti-SLC13A2 (IP:21722-1-AP, 4ug; Detection:21722-1-AP 1:300) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human kidney using 21722-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21722-1-AP (SLC13A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 21722-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21722-1-AP (SLC13A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 3 publications below

Product Information

The immunogen of 21722-1-AP is SLC13A2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC13A2 fusion protein Ag16379
• Full Name: solute carrier family 13 (sodium-dependent dicarboxylate transporter), member 2
• Calculated Molecular Weight: 592 aa, 64 kDa
• GenBank Accession Number: BC096277
• Gene Symbol: SLC13A2
• Gene ID (NCBI): 9058
• RRID: AB_2878912
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Am J Physiol Renal Physiol; Expression of sodium-dependent dicarboxylate transporter 1 (NaDC1/SLC13A2) in normal and neoplastic human kidney.; Authors - Hyun-Wook Lee
human	IHC	Cancer Med; Transcriptome analysis in primary colorectal cancer tissues from patients with and without liver metastases using next-generation sequencing.; Authors - Sen Wang
mouse		Am J Physiol Renal Physiol; Regulation of Renal NaDC1 Expression and Citrate Excretion by NBCe1-A.; Authors - Gunars Osis
mouse	IHC	Physiol Rep; Effect of NBCe1 deletion on renal citrate and 2-oxoglutarate handling.; Authors - Gunars Osis ; KO Validated