Tested Applications
| Positive WB detected in | COLO 320 cells, Caco-2 cells, HT-29 cells, MOLT-4 cells, mouse small intestine tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21726-1-AP targets SLC15A1/PEPT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16390 Product name: Recombinant human SLC15A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 374-580 aa of BC096327 Sequence: VAAIVQVEIDKTLPVFPKGNEVQIKVLNIGNNTMNISLPGEMVTLGPMSQTNAFMTFDVNKLTRINISSPGSPVTAVTDDFKQGQRHTLLVWAPNHYQVVKDGLNQKPEKGENGIRFVNTFNELITITMSGKVYANISSYNASTYQFFPSGIKGFTISSTEIPPQCQPNFNTFYLEFGSAYTYIVQRKNDSCPEVKVFEDISANTVN Predict reactive species |
| Full Name | solute carrier family 15 (oligopeptide transporter), member 1 |
| Calculated Molecular Weight | 708 aa, 79 kDa |
| Observed Molecular Weight | 70-80 kDa |
| GenBank Accession Number | BC096327 |
| Gene Symbol | SLC15A1/PEPT1 |
| Gene ID (NCBI) | 6564 |
| RRID | AB_3085668 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P46059 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SLC15A1/PEPT1 is a peptide transporter that transports oligopeptides of 2 to 4 amino acids with a preference for dipeptides. PEPT1 mediates transepithelial transport of muramyl and N-formylated bacterial dipeptides contributing to recognition of pathogenic bacteria by the mucosal immune system. PEPT1 is primarily expressed in the small intestine (PMID: 22194420). PEPT1 is also expressed in distal colon in rodents and humans and contributes to water absorption. 71 kDa nonglycosylated PEPT1 protein can be detected (PMID: 23660505).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC15A1/PEPT1 antibody 21726-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SLC15A1/PEPT1 Polyclonal antibody (Cat# 21726-1-AP) has 5 total citations. These appear in Nature Communications, Diabetes & Obesity Metabolism, Journal of the American Heart Association, Photodiagnosis and Photodynamic Therapy, and Journal of Oral Biosciences. The associated research fields span pharmacology (oral drug absorption), diabetology (renal microcirculation), cardiology (cardiac fibrosis), oncology (gastrointestinal carcinoma diagnosis), and cell biology (peptide transporter regulation).
| Species | Application | Title |
|---|---|---|
Nat Commun Engineered vesicles enhance oral antibiotic absorption in proximal small intestine and mitigate gut dysbiosis. | ||
Diabetes Obes Metab Rapid improvement of renal microcirculatory homeostasis by liraglutide following diabetes induction. | ||
J Am Heart Assoc Stanniocalcin-1 Attenuates Cardiac Fibrosis Post Ischemia-Reperfusion Injury by Targeting the DOCKER Domain of Dedicator of Cytokinesis 1. | ||
Photodiagnosis Photodyn Ther Expression of coproporphyrinogen oxidase is associated with detection of upper gastrointestinal carcinomas by 5-aminolevulinic acid-mediated photodynamic diagnosis. | ||
J Oral Biosci RANKL enhances the expression of PEPT1/SLC15A1 and PEPT2/SLC15A2 in RAW264.7 cells. |
