Filter: AllWBIHC

WB analysis of mouse kidney using 20553-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20553-1-AP (SLC16A12 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 20553-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20553-1-AP (SLC16A12 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
IHC staining of human stomach cancer using 20553-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20553-1-AP (SLC16A12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 20553-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20553-1-AP (SLC16A12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 20553-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20553-1-AP (SLC16A12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 20553-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20553-1-AP (SLC16A12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 20553-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20553-1-AP (SLC16A12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 20553-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 20553-1-AP (SLC16A12 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 20553-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 20553-1-AP (SLC16A12 antibody) at dilution of 1:50 (under 40x lens).

SLC16A12 Polyclonal antibody

Name: SLC16A12 Polyclonal antibody
Brand: Proteintech
SKU: 20553-1-AP

SLC16A12 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 20553-1-AP

Publications(2)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Synonyms

CJMG, Creatine transporter 2, CRT2, MCT 12, MCT12

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information
Publications (2)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse kidney tissue
Positive IHC detected in	human stomach cancer tissue, human kidney tissue, human testis tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 1 publications below

Product Information

20553-1-AP targets SLC16A12 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, xenopus
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	solute carrier family 16, member 12 (monocarboxylic acid transporter 12)
Calculated Molecular Weight	53 kDa
Observed Molecular Weight	53-56 kDa
GenBank Accession Number	NM_213606
Gene Symbol	SLC16A12
Gene ID (NCBI)	387700
RRID	AB_10693621
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6ZSM3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC16A12, also named as MCT12 and CJMG, belongs to the major facilitator superfamily and Monocarboxylate porter (TC 2.A.1.13) family. It is a proton-linked monocarboxylate transporter. SLC16A12 catalyzes the rapid transport across the plasma membrane of many monocarboxylates. The antibody is specific to SLC16A12.

Protocols

Product Specific Protocols
IHC protocol for SLC16A12 antibody 20553-1-AP	Download protocol
WB protocol for SLC16A12 antibody 20553-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
Xenopus	WB	Biochem Biophys Res Commun Molecular characterization of the orphan transporter SLC16A9, an extracellular pH- and Na+-sensitive creatine transporter. Authors - Yuya Futagi View Article
human	IHC	Medicine (Baltimore) Decreased expression of SLC16A12 mRNA predicts poor prognosis of patients with clear cell renal cell carcinoma. Authors - Jie Mei View Article