SLC16A12 Polyclonal antibody

SLC16A12 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20553-1-AP

Synonyms

Monocarboxylate transporter 12, MCT12, MCT 12, CRT2, Creatine transporter 2

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Validation Data Gallery

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  WB analysis of mouse kidney using 20553-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20553-1-AP (SLC16A12 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20553-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20553-1-AP (SLC16A12 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 20553-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20553-1-AP (SLC16A12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 20553-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20553-1-AP (SLC16A12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 20553-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20553-1-AP (SLC16A12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 20553-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20553-1-AP (SLC16A12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 20553-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20553-1-AP (SLC16A12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 20553-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20553-1-AP (SLC16A12 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 20553-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20553-1-AP (SLC16A12 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse kidney tissue
• Positive IHC detected in: human stomach cancer tissue, mouse testis tissue, human kidney tissue, human testis tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

20553-1-AP targets SLC16A12 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, xenopus
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: solute carrier family 16, member 12 (monocarboxylic acid transporter 12)
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 53-56 kDa
• GenBank Accession Number: NM_213606
• Gene Symbol: SLC16A12
• Gene ID (NCBI): 387700
• RRID: AB_10693621
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC16A12, also named as MCT12 and CJMG, belongs to the major facilitator superfamily and Monocarboxylate porter (TC 2.A.1.13) family. It is a proton-linked monocarboxylate transporter. SLC16A12 catalyzes the rapid transport across the plasma membrane of many monocarboxylates. The antibody is specific to SLC16A12.

Publications

Species	Application	Title
Xenopus	WB	Biochem Biophys Res Commun; Molecular characterization of the orphan transporter SLC16A9, an extracellular pH- and Na+-sensitive creatine transporter.; Authors - Yuya Futagi
human	IHC	Medicine (Baltimore); Decreased expression of SLC16A12 mRNA predicts poor prognosis of patients with clear cell renal cell carcinoma.; Authors - Jie Mei