SLC16A2 Polyclonal antibody

SLC16A2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 20676-1-AP

Synonyms

AHDS, DXS128, DXS128E, MCT 7, MCT 8, MCT7, MCT8, Monocarboxylate transporter 7, Monocarboxylate transporter 8, MRX22, SLC16A2, XPCT

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Validation Data Gallery

• 

  WB analysis of mouse kidney using 20676-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20676-1-AP (SLC16A2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 20676-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20676-1-AP (SLC16A2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 20676-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20676-1-AP (SLC16A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 20676-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20676-1-AP (SLC16A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse kidney tissue, L02 cells, mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below

Product Information

20676-1-AP targets SLC16A2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: solute carrier family 16, member 2 (monocarboxylic acid transporter 8)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 50-65 kDa
• GenBank Accession Number: NM_006517
• Gene Symbol: SLC16A2
• Gene ID (NCBI): 6567
• RRID: AB_10695188
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC16A2, also named as MCT8 and XPCT, belongs to the major facilitator superfamily and Monocarboxylate porter (TC 2.A.1.13) family. SLC16A2 is a very active and specific thyroid hormone transporter. SLC16A2 stimulates cellular uptake of thyroxine (T4), triiodothyronine (T3), reverse triiodothyronine (rT3) and diidothyronine. It does not transport Leu, Phe, Trp or Tyr. Defects in SLC16A2 are the cause of monocarboxylate transporter 8 deficiency (MCT8 deficiency). This antibody is specific to SLC16A2.

Publications

Species	Application	Title
mouse	WB	Int J Mol Sci; Smoothelin-Like Protein 1 Regulates the Thyroid Hormone-Induced Homeostasis and Remodeling of C2C12 Cells via the Modulation of Myosin Phosphatase.; Authors - Evelin Major
human	WB	Front Pharmacol; Hydrogen Sulfide Promotes Thyroid Hormone Synthesis and Secretion by Upregulating Sirtuin-1.; Authors - Xue Zhao
mouse	WB	PLoS One; Tissue-Specific Expression of Monocarboxylate Transporters during Fasting in Mice.; Authors - Alexandra Schutkowski
rat	WB	Pharm Res; Participation of Monocarboxylate Transporter 8, But Not P-Glycoprotein, in Carrier-Mediated Cerebral Elimination of Phenytoin across the Blood-Brain Barrier.; Authors - Ryuta Jomura
human	WB	Toxicology; MiR-24-3p/Dio3 axis is essential for BDE47 to induce local thyroid hormone disorder and neurotoxicity; Authors - Min Liu
human	WB	Toxicology; BDE209-promoted Dio2 degradation in H4 glioma cells through the autophagy pathway, resulting in hypothyroidism and leading to neurotoxicity; Authors - Min Liu