MCT2 Polyclonal antibody

MCT2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 20355-1-AP

Synonyms

MCT2, SLC16A7

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 20355-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20355-1-AP (MCT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 20355-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20355-1-AP (MCT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 20355-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20355-1-AP (MCT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 20355-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20355-1-AP (MCT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, HEK-293 cells, K-562 cells
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below

Product Information

20355-1-AP targets MCT2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MCT2 fusion protein Ag14201
• Full Name: solute carrier family 16, member 7 (monocarboxylic acid transporter 2)
• Calculated Molecular Weight: 478 aa, 52 kDa
• Observed Molecular Weight: 40-52 kDa
• GenBank Accession Number: BC030693
• Gene Symbol: SLC16A7
• Gene ID (NCBI): 9194
• RRID: AB_2878680
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC16A7, also named MCT2, is a proton-coupled monocarboxylate transporter catalyzing the rapid transport across the plasma membrane of many monocarboxylates. It has been reported that SLC16A7 is the primary monocarboxylate transporter in Purkinje cells and can be detected in the brain, cerebellum, liver, and kidney, but not in vascular smooth muscle cells, pericytes, endothelium, heart, lung, or stomach. There are some isoforms of SLC16A7 with bands around 52 kDa and 40-45 kDa in SDS-PAGE. The 80 kDa protein might be the dimer similar to the papers published. (PMID: 9482213, 15749979, 11291733, 16901736)

Publications

Species	Application	Title
human	WB	Br J Cancer; Autoregulation of H+/lactate efflux prevents monocarboxylate transport (MCT) inhibitors from reducing glycolytic lactic acid production.; Authors - Wiktoria Blaszczak
rat	WB, IF	Anim Cells Syst (Seoul); MCT2 overexpression promotes recovery of cognitive function by increasing mitochondrial biogenesis in a rat model of stroke.; Authors - Xiaorong Yu
human	WB	J Exp Clin Cancer Res; ROS/PI3K/Akt and Wnt/β-catenin signalings activate HIF-1α-induced metabolic reprogramming to impart 5-fluorouracil resistance in colorectal cancer.; Authors - Shuohui Dong
mouse	WB	Biomed Pharmacother; Fibroblast growth factor 21 protects the liver from apoptosis in a type 1 diabetes mouse model via regulating L-lactate homeostasis; Authors - Jiapin Yan