EAAT2 Polyclonal antibody

EAAT2 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22515-1-AP

Synonyms

EAAT2, GLT 1, GLT1, SLC1A2



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22515-1-AP targets EAAT2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EAAT2 fusion protein Ag18247
Full Name solute carrier family 1 (glial high affinity glutamate transporter), member 2
Calculated Molecular Weight 574 aa, 62 kDa
Observed Molecular Weight 60-70 kDa, 140 kDa
GenBank Accession NumberBC132768
Gene Symbol SLC1A2
Gene ID (NCBI) 6506
RRIDAB_2879112
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EAAT2 (also known as GLT-1), is encoded by SLC1A2 and is a glutamate transporter which can clear the majority of extracellular glutamate in brain and prevent brain seizures.Three isoforms of EAAT2 exist due to the alternative splicing. In addition to the 65-70 kDa monomer form, 130-150 kDa dimer of EAAT2 can also be observed on SDS-PAGE. (PMID: 22114258)

Protocols

Product Specific Protocols
WB protocol for EAAT2 antibody 22515-1-APDownload protocol
IP protocol for EAAT2 antibody 22515-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Theranostics

Hsp90 inhibitor HSP990 in very low dose upregulates EAAT2 and exerts potent antiepileptic activity.

Authors - Longze Sha
mouseIF

Oxid Med Cell Longev

Photobiomodulation Therapy Ameliorates Glutamatergic Dysfunction in Mice with Chronic Unpredictable Mild Stress-Induced Depression.

Authors - Di Zhang
mouseWB

Aging Cell

HIV and drug abuse mediate astrocyte senescence in a β-catenin-dependent manner leading to neuronal toxicity.

Authors - Chunjiang Yu
pigWB

Neural Regen Res

Astrocyte-neuron communication mediated by the Notch signaling pathway: focusing on glutamate transport and synaptic plasticity

Authors - Ke-Xin Li
mouseWB

Oxid Med Cell Longev

Neuroprotective Effect of Ceftriaxone on MPTP-Induced Parkinson's Disease Mouse Model by Regulating Inflammation and Intestinal Microbiota

Authors - Xiaoting Zhou
humanWB

J Proteomics

Quantitative proteomic analysis of cortex in the depressive-like behavior of rats induced by the simulated complex space environment.

Authors - Rui Min

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Azita (Verified Customer) (05-31-2021)

The cells were subjected to SDS PAGE followed by WB and the EAAT2 antibody was used at 1:1000 dilution and was incubated overnight in 4°C.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Human primary cortical cells