EAAT2 Polyclonal antibody

EAAT2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 22515-1-AP

Synonyms

EAAT2, GLT 1, GLT1, SLC1A2

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Validation Data Gallery

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  WB analysis of mouse brain using 22515-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22515-1-AP (EAAT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 22515-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 22515-1-AP (EAAT2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 22515-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 22515-1-AP (EAAT2 antibody) at dilution of 1:50 (under 40x lens).

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  IP experiment of mouse brain using 22515-1-AP

  IP Result of anti-EAAT2 (IP:22515-1-AP, 3ug; Detection:22515-1-AP 1:1000) with mouse brain tissue lysate 6000ug.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 7 publications below

Product Information

The immunogen of 22515-1-AP is EAAT2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EAAT2 fusion protein Ag18247
• Full Name: solute carrier family 1 (glial high affinity glutamate transporter), member 2
• Calculated molecular weight: 574 aa, 62 kDa
• Observed molecular weight: 60-70 kDa, 140 kDa
• GenBank accession number: BC132768
• Gene symbol: SLC1A2
• Gene ID (NCBI): 6506
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

EAAT2 (also known as GLT-1), is encoded by SLC1A2 and is a glutamate transporter which can clear the majority of extracellular glutamate in brain and prevent brain seizures.Three isoforms of EAAT2 exist due to the alternative splicing. In addition to the 65-70 kDa monomer form, 130-150 kDa dimer of EAAT2 can also be observed on SDS-PAGE. (PMID: 22114258)

Publications

Species	Application	Title
mouse	WB	Neurochem Res; Aucubin Alleviates Seizures Activity in Li-Pilocarpine-Induced Epileptic Mice: Involvement of Inhibition of Neuroinflammation and Regulation of Neurotransmission.; Authors - Siyu Chen
mouse	WB	Aging (Albany NY); Ginsenoside Rb1 prevents MPTP-induced changes in hippocampal memory via regulation of the α-synuclein/PSD-95 pathway.; Authors - Shaogang Qu
mouse	WB	Cell Death Dis; Functional validation of a human GLUD2 variant in a murine model of Parkinson's disease.; Authors - Wenlong Zhang
rat	WB	Neural Regen Res; Baicalin protects neonatal rat brains against hypoxic-ischemic injury by upregulating glutamate transporter 1 via the phosphoinositide 3-kinase/protein kinase B signaling pathway.; Authors - Zhi-Qing Zhou
rat	WB	Brain Res Bull; PMID: 32621862; Authors - Fang Yang
mouse	WB	Aging Cell; HIV and drug abuse mediate astrocyte senescence in a β-catenin-dependent manner leading to neuronal toxicity.; Authors - Chunjiang Yu