SLC1A4 Polyclonal antibody

SLC1A4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 13067-2-AP

Synonyms

ASCT 1, ASCT1, SATT, SLC1A4

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Validation Data Gallery

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  WB analysis using 13067-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13067-2-AP (SLC1A4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13067-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13067-2-AP (SLC1A4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 13067-2-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13067-2-AP (SLC1A4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 13067-2-AP

  IP result of anti-SLC1A4 (IP:13067-2-AP, 4ug; Detection:13067-2-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse kidney using 13067-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13067-2-AP (SLC1A4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 13067-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13067-2-AP (SLC1A4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 13067-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13067-2-AP (SLC1A4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13067-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13067-2-AP (SLC1A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Raji cells, mouse brain tissue, human skeletal muscle tissue, SW480 cells, mouse kidney tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse kidney tissue, human kidney tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below

Product Information

13067-2-AP targets SLC1A4 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC1A4 fusion protein Ag3763
• Full Name: solute carrier family 1 (glutamate/neutral amino acid transporter), member 4
• Calculated Molecular Weight: 532 aa, 56 kDa
• Observed Molecular Weight: 62-70 kDa
• GenBank Accession Number: BC026216
• Gene Symbol: SLC1A4
• Gene ID (NCBI): 6509
• RRID: AB_2190604
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Cancer; CircMYH9 drives colorectal cancer growth by regulating serine metabolism and redox homeostasis in a p53-dependent manner; Authors - Xin Liu
mouse	WB	Ecotoxicol Environ Saf; Toxic effects of maternal cadmium exposure on the metabolism and transport system of amino acids in the maternal livers; Authors - Peng Xu
human	WB	Front Oncol; SLC1A4: A Powerful Prognostic Marker and Promising Therapeutic Target for HCC.; Authors - Xiaozhen Peng
mouse	WB	Res Sq; Generation and characterization of a knock-in mouse model for Spastic Tetraplegia, Thin Corpus Callosum, and Progressive Microcephaly (SPATCCM); Authors - Megan L Ratz
mouse	WB	Mamm Genome; Generation and characterization of a knock-in mouse model for spastic tetraplegia, thin corpus callosum, and progressive microcephaly (SPATCCM); Authors - Megan L Ratz-Mitchem ; KO Validated