SLC20A2 Polyclonal antibody

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SLC20A2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 12820-1-AP Copied!

Publications (14)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF-P, IP, ELISA

Synonyms

Gibbon ape leukemia virus receptor 2, GLVR2, GLVR-2, hPit2, Phosphate transporter 2

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  WB analysis using 12820-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12820-1-AP (SLC20A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of COLO 320 using 12820-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12820-1-AP (SLC20A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of COLO 320 using 12820-1-AP

  IP result of anti-SLC20A2 (IP:12820-1-AP, 4ug; Detection:12820-1-AP 1:400) with COLO 320 cells lysate 2400ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human bowen disease using 12820-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 12820-1-AP (SLC20A2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human bowen disease using 12820-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 12820-1-AP (SLC20A2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 12820-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12820-1-AP (SLC20A2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 12820-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12820-1-AP (SLC20A2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse brain using 12820-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SLC20A2 antibody (12820-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse brain tissue, COLO 320 cells, mouse kidney tissue
• Positive IP detected in: COLO 320 cells
• Positive IHC detected in: human bowen disease, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12820-1-AP targets SLC20A2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3469

  Product name: Recombinant human SLC20A2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 231-512 aa of BC028600

  Sequence: WLFVCPWMRRKITGKLQKEGALSRVSDESLSKVQEAESPVFKELPGAKANDDSTIPLTGAAGETLGTSEGTSAGSHPRAAYGRALSMTHGSVKSPISNGTFGFDGHTRSDGHVYHTVHKDSGLYKDLLHKIHIDRGPEEKPAQESNYRLLRRNNSYTCYTAAICGLPVHATFRAADSSAPEDSEKLVGDTVSYSKKRLRYDSYSSYCNAVAEAEIEAEEGGVEMKLASELADPDQPREDPAEEEKEEKDAPEVHLLFHFLQVLTACFGSFAHGGNDVSNAIG

• Full Name: solute carrier family 20 (phosphate transporter), member 2
• Calculated Molecular Weight: 652 aa, 70 kDa
• Observed Molecular Weight: 68-70 kDa
• GenBank Accession Number: BC028600
• Gene Symbol: SLC20A2
• Gene ID (NCBI): 6575
• RRID: AB_2191004
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q08357
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC20A2 (also known as PiT2) is a sodium (Na)-phosphate (Pi) co-transporter that belongs to the SLC20 family. SLC20A2 is highly expressed in the central nervous system and controls hippocampal-dependent learning and memory (PMID: 38195526). SLC20A2 is the major causative gene for primary familial brain calcification (PFBC), a rare neurological disease characterized by widespread cognitive impairment.

Publications

What published studies show

SLC20A2 Polyclonal Antibody (Cat# 12820-1-AP) has 14 citations across journals including Cell Death & Disease, Advanced Healthcare Materials, Arteriosclerosis Thrombosis and Vascular Biology, Movement Disorders, Cell Reports, British Journal of Pharmacology, Nutrients, Journal of Biological Chemistry, Bone, Endocrinology, and Journal of Applied Toxicology. Research fields span phosphate metabolism, bone mineralization/osteogenesis, valvular calcification, neuronal plasticity and cognition, primary familial brain calcification, hypophosphatemia, chronic kidney disease, and cadmium-induced renal injury.

Automatically generated from the 14 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Cell Death Dis; Slc20a1 and Slc20a2 regulate neuronal plasticity and cognition independently of their phosphate transport ability; Authors - Mariana Ramos-Brossier
human	WB	Adv Healthc Mater; Correlating Material Properties to Osteoprotegerin Expression on Nanoparticulate Mineralized Collagen Glycosaminoglycan Scaffolds; Authors - Wei Chen ; KD Validated
human	WB	Adv Healthc Mater; Modulating Temporospatial Phosphate Equilibrium by Nanoparticulate Mineralized Collagen Materials Induces Osteogenesis via PiT-1 and PiT-2; Authors - Xiaoyan Ren
human	WB	Arterioscler Thromb Vasc Biol; Potential Role of H-Ferritin in Mitigating Valvular Mineralization.; Authors - Katalin Éva Sikura
human	WB	Mov Disord; A Homozygous Variant in NAA60 Is Associated with Primary Familial Brain Calcification; Authors - Xinhui Chen
human	WB	Cell Rep; Homeostatic coordination of cellular phosphate uptake and efflux requires an organelle-based receptor for the inositol pyrophosphate IP8; Authors - Xingyao Li