SLC22A16 Polyclonal antibody

SLC22A16 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 26833-1-AP

Synonyms

Carnitine transporter 2, CT2, Flipt 2, FLIPT2, OCT6, OKB1, SLC22A16

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Validation Data Gallery

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  WB analysis using 26833-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26833-1-AP (SLC22A16 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 26833-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26833-1-AP (SLC22A16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 26833-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26833-1-AP (SLC22A16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 26833-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26833-1-AP (SLC22A16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 26833-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26833-1-AP (SLC22A16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of THP-1 using 26833-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using SLC22A16 antibody (26833-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells, PC-3 cells
• Positive IHC detected in: human breast cancer tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26833-1-AP targets SLC22A16 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC22A16 fusion protein Ag25270
• Full Name: solute carrier family 22 (organic cation/carnitine transporter), member 16
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC047565
• Gene Symbol: SLC22A16
• Gene ID (NCBI): 85413
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC22A16, also named as CT2, FLIPT2, or OCT6, belongs to the major facilitator (TC 2.A.1) superfamily and organic cation transporter (TC 2.A.1.19) family. SLC22A16 encodes an organic zwitterion transporter protein that transports carnitine and some anticancer drugs, including anthracyclines. As a carnitine transporter, SLC22A16 is involved in the regulation of fatty acid oxidation (FAO), which plays an important role also in the metabolic transformation of cancer cells. SLC22A16 expression in cancer cells is associated with increased sensitivity to the cytotoxic effects of doxorubicin. SLC22A16 has 3 isoforms with the molecular mass of 58, 61 and 65 kDa (PMID: 35761855, 35608746).