SLC22A18 Polyclonal antibody

• Featured Product
• KD/KO Validated

SLC22A18 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 11646-2-AP

Synonyms

BWR1A, BWSCR1A, HET, IMPT1, ITM, ORCTL 2, ORCTL2, p45 BWR1A, SLC22A18, SLC22A1L, TSSC5

---

Validation Data Gallery

• 

  WB analysis of mouse brain using 11646-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11646-2-AP (SLC22A18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 11646-2-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11646-2-AP (SLC22A18 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11646-2-AP

  IP result of anti-SLC22A18 (IP:11646-2-AP, 4ug; Detection:11646-2-AP 1:600) with mouse brain tissue lysate 4000ug.

---

Tested Applications

• Positive WB detected in: mouse brain tissue, COLO 320 cells
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

11646-2-AP targets SLC22A18 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC22A18 fusion protein Ag2245
• Full Name: solute carrier family 22, member 18
• Calculated Molecular Weight: 408 aa, 43 kDa
• Observed Molecular Weight: 40-50 kDa
• GenBank Accession Number: BC015571
• Gene Symbol: SLC22A18
• Gene ID (NCBI): 5002
• RRID: AB_2191146
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC22A18 is a member of the family of polyspecific organic-cation transporters that localize at the apical membrane surface of renal proximal tubules. SLC22A18 is expressed at high levels in the kidney, liver, colon and in fetal renal proximal tubules and present at lower levels in heart, brain and lung. Defects in SLC22A18 are associated with breast and lung cancer and are the cause of rhabdomyosarcoma type 1, a malignant tumor derived from striated muscle. More recently, SLC22A18 has been shown to functions as a tumor suppressor in glioma and represents a candidate biomarker for long-term survival in this disease.

Publications

Species	Application	Title
human	WB	Int J Biol Macromol; microRNA-137 functions as a tumor suppressor in human non-small cell lung cancer by targeting SLC22A18.; Authors - Bicheng Zhang ; KD Validated
		Hum Mol Genet; Parent-of-origin tumorigenesis is mediated by an essential imprinted modifier in SDHD-linked paragangliomas: SLC22A18 and CDKN1C are candidate tumor modifiers.; Authors - Attje S Hoekstra
human	IHC	Cancer Epidemiol; Low expression of SLC22A18 predicts poor survival outcome in patients with breast cancer after surgery.; Authors - He Hongyu H