Tested Applications
| Positive WB detected in | mouse brain tissue, mouse heart tissue, rat brain tissue, rat heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27591-1-AP targets SLC24A3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26297 Product name: Recombinant human SLC24A3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 361-450 aa of NM_020689 Sequence: MSRAYTNGESEVAIKIPIKHTVENGTGPSSAPDRGVNGTRRDDVVAEAGNETENENEDNENDEEEEEDEDDDEGPYTPFDTPSGKLETVKW Predict reactive species |
| Full Name | solute carrier family 24 (sodium/potassium/calcium exchanger), member 3 |
| Calculated Molecular Weight | 72 kDa |
| Observed Molecular Weight | 50-60 kDa |
| GenBank Accession Number | NM_020689 |
| Gene Symbol | SLC24A3 |
| Gene ID (NCBI) | 57419 |
| RRID | AB_3742241 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HC58 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SLC24A3, also known as NCKX3 (Na⁺/K⁺/Ca²⁺ exchanger 3), is a member of the solute carrier family 24 (SLC24), which comprises five K⁺-dependent Na⁺/Ca²⁺ exchangers (NCKX1-5). These transporters are critical for maintaining intracellular calcium homeostasis by coupling the extrusion of one Ca²⁺ ion and one K⁺ ion to the influx of four Na⁺ ions. SLC24A3 plays a key role in calcium signaling and homeostasis, influencing processes such as neurotransmission, smooth muscle contraction, and female reproductive physiology. Relatively high levels of SLC24A3 expression can be observed in heart tissue, consistent with the expression patterns reported in the literature. The observed molecular weight of SLC24A3 is 50-60 kDa, which is consistent with the literature description. (PMID: 21573014, PMID: 28588505)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC24A3 antibody 27591-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
