SLC25A11 Polyclonal antibody

• Featured Product
• KD/KO Validated

SLC25A11 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12253-1-AP

Synonyms

SLC20A4, OGCP, OGC, Mitochondrial 2-oxoglutarate/malate carrier protein, M2OM

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Validation Data Gallery

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  WB analysis using 12253-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12253-1-AP (SLC25A11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12253-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12253-1-AP (SLC25A11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12253-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12253-1-AP (SLC25A11 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 12253-1-AP

  IP result of anti-SLC25A11 (IP:12253-1-AP, 4ug; Detection:12253-1-AP 1:1000) with HEK-293 cells lysate 1560ug.

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  IHC staining of human stomach cancer using 12253-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12253-1-AP (SLC25A11 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 12253-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 12253-1-AP (SLC25A11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, human kidney tissue, HEK-293 cells, Raji cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

12253-1-AP targets SLC25A11 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC25A11 fusion protein Ag2903
• Full Name: solute carrier family 25 (mitochondrial carrier; oxoglutarate carrier), member 11
• Calculated Molecular Weight: 314 aa, 34 kDa
• Observed Molecular Weight: 28-34 kDa
• GenBank Accession Number: BC016294
• Gene Symbol: SLC25A11
• Gene ID (NCBI): 8402
• RRID: AB_2877840
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF,IP	EMBO Rep; LINC00493-encoded microprotein SMIM26 exerts anti-metastatic activity in renal cell carcinoma; Authors - Kun Meng ; KD Validated
human,mouse	WB,IF	Proc Natl Acad Sci U S A; Mitochondrial outer membrane protein FUNDC2 promotes ferroptosis and contributes to doxorubicin-induced cardiomyopathy; Authors - Na Ta ; KO Validated
human	WB	Science; Autoregulatory control of mitochondrial glutathione homeostasis; Authors - Yuyang Liu