SLC25A18 Polyclonal antibody

SLC25A18 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 17348-1-AP

Synonyms

GC 2, GC2, Glutamate/H(+) symporter 2, SLC25A18

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Validation Data Gallery

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  WB analysis using 17348-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17348-1-AP (SLC25A18 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 17348-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17348-1-AP (SLC25A18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17348-1-AP

  IP result of anti-SLC25A18 (IP:17348-1-AP, 4ug; Detection:17348-1-AP 1:500) with mouse brain tissue lysate 4400ug.

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  IHC staining of mouse brain using 17348-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17348-1-AP (SLC25A18 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17348-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17348-1-AP (SLC25A18 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse liver tissue, mouse kidney tissue, mouse heart tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

17348-1-AP targets SLC25A18 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC25A18 fusion protein Ag11420
• Full Name: solute carrier family 25 (mitochondrial carrier), member 18
• Calculated Molecular Weight: 315 aa, 34 kDa
• Observed Molecular Weight: 32-35 kDa
• GenBank Accession Number: BC031644
• Gene Symbol: SLC25A18
• Gene ID (NCBI): 83733
• RRID: AB_2189889
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC25A18, also named as GC 2, belongs to the mitochondrial transporter family (SLC25). It is located in inner mitochondrial membrane which involved in the transport of glutamate either with H⁺ or in exchange for OH^-. SLC25A18 is highly expressed in brain, testis, lung, kidney, liver, spleen and skeletal muscle. 17348-1-AP antibody detects the band around 32-35 kDa in SDS-PAGE.   (PMID: 14598172)

Publications

Species	Application	Title
human	IHC	Am J Cancer Res; SLC25A18 has prognostic value in colorectal cancer and represses Warburg effect and cell proliferation via Wnt signaling.; Authors - Li Liang
mouse	WB,IF	Mitochondrion; Increased expression of SLC25A18 is associated with Alzheimer's disease and is involved in Aβ42-induced mitochondrial dysfunction and apoptosis in neuronal cells; Authors - Jia-Yi Song
mouse	WB	MedComm (2020); Nicotinamide mononucleotide supplementation rescues mitochondrial and energy metabolism functions and ameliorates inflammatory states in the ovaries of aging mice; Authors - Jinghui Liang