SLC25A23 Polyclonal antibody

SLC25A23 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20168-1-AP

Synonyms

APC2, MCSC2, SCaMC 3, SCAMC3, SLC25A23

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Validation Data Gallery

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  WB analysis of mouse pancreas using 20168-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 20168-1-AP (SLC25A23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 20168-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20168-1-AP (SLC25A23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 20168-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20168-1-AP (SLC25A23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 20168-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20168-1-AP (SLC25A23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 20168-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20168-1-AP (SLC25A23 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas using 20168-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20168-1-AP (SLC25A23 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse pancreas tissue, mouse skeletal muscle tissue, mouse testis tissue, SH-SY5Y cells
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

The immunogen of 20168-1-AP is SLC25A23 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC25A23 fusion protein Ag13987
• Full Name: solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 23
• Calculated Molecular Weight: 468 aa, 52 kDa
• Observed Molecular Weight: 49-54 kDa
• GenBank Accession Number: BC001656
• Gene Symbol: SLC25A23
• Gene ID (NCBI): 79085
• RRID: AB_10638608
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Biomed Res Int; Comprehensive Analysis and Validation of Solute Carrier Family 25 (SLC25) and Its Correlation with Immune Infiltration in Pan-Cancer; Authors - Ao-ran Liu