SLC25A23 Polyclonal antibody

SLC25A23 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20168-1-AP

Synonyms

SCAMC3, SCaMC 3, Mitochondrial ATP-Mg/Pi carrier protein 2, Mitochondrial adenyl nucleotide antiporter SLC25A23, MCSC2

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Validation Data Gallery

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  WB analysis of mouse pancreas using 20168-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 20168-1-AP (SLC25A23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 20168-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20168-1-AP (SLC25A23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 20168-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20168-1-AP (SLC25A23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 20168-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20168-1-AP (SLC25A23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 20168-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20168-1-AP (SLC25A23 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas using 20168-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 20168-1-AP (SLC25A23 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse pancreas tissue, mouse skeletal muscle tissue, mouse testis tissue, SH-SY5Y cells
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

20168-1-AP targets SLC25A23 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC25A23 fusion protein Ag13987
• Full Name: solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 23
• Calculated Molecular Weight: 468 aa, 52 kDa
• Observed Molecular Weight: 49-54 kDa
• GenBank Accession Number: BC001656
• Gene Symbol: SLC25A23
• Gene ID (NCBI): 79085
• RRID: AB_10638608
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC25A23 gene encodes a 468 amino acids polypeptide, named SCaMC-3, with a bipartite structure typical of calcium-binding mitochondrial solute carrier (CaMSC) proteins. The amino-terminal portion harbors three canonical EF-hand calcium-binding domains while the carboxyl-terminal portion of SCaMC-3 has the characteristic features of the mitochondrial solute carrier (MSC) superfamily. Northern blot analysis reveals the presence of the transcript in brain, heart, skeletal muscle, liver and small intestine. The SLC25A23 gene undergoes alternative splicing suggesting a modular nature of the encoded product. Three out of four putative protein isoforms lack a significant portion of the third mitochondrial carrier signature. The most common SCaMC-3 isoform shows a mitochondrial subcellular localization when transfected in HeLa cells and is able to bind calcium by Ca2+ dependent mobility shift assays.(PMID: 15716113)

Publications

Species	Application	Title
human	IHC	Biomed Res Int; Comprehensive Analysis and Validation of Solute Carrier Family 25 (SLC25) and Its Correlation with Immune Infiltration in Pan-Cancer; Authors - Ao-ran Liu