SLC25A29 Polyclonal antibody

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  WB analysis using 26663-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26663-1-AP (SLC25A29 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 26663-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26663-1-AP (SLC25A29 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 26663-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26663-1-AP (SLC25A29 antibody) at dilution of 1:200 (under 40x lens).

SLC25A29 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 26663-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

C14orf69, CACT-like, Carnitine/acylcarnitine translocase-like, Mitochondrial basic amino acids transporter, Mitochondrial carnitine/acylcarnitine carrier protein CACL

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

26663-1-AP targets SLC25A29 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag24662

  Product name: Recombinant human SLC25A29 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-146 aa of BC132966

  Sequence: MGLTFINALVFGVQGNTLRALGHDSPLNQFLAGAAAGAIQCVICCPMELAKTRLQLQDAGPARTYKGSLDCLAQIYGHEGLRGVNRGMVSTLLRETPSFGVYFLTYDALTRALGCEPGDRLLVPKLLLAGGTSGIVSWLSTYPVDV

• Full Name: solute carrier family 25, member 29
• Calculated Molecular Weight: 303 aa, 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC132966
• Gene Symbol: SLC25A29
• Gene ID (NCBI): 123096
• RRID: AB_2880593
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N8R3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC25A29 is a mitochondrial transporter belonging to the SLC25 superfamily, which facilitates the exchange of metabolites across the inner mitochondrial membrane. Initially reported as a carnitine/acylcarnitine-like carrier, it is now recognized as a key amino acid transporter, particularly for arginine and lysine, contributing to mitochondrial protein synthesis and amino acid degradation. SLC25A29 is primarily expressed in the heart, brain, liver, and kidneys and is associated with metabolic disorders and vascular diseases. Its expression is regulated by histone lactose modification and is associated with conditions such as lung adenocarcinoma and diabetic retinopathy.(PMID: 24652292; 37775731;29459713; 40244673)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cancer Cell Int; Comprehensive bioinformatics analysis of the solute carrier family and preliminary exploration of SLC25A29 in lung adenocarcinoma; Authors - Pengdou Zheng