SLC25A31/ANT4 Polyclonal antibody

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  WB analysis using 33263-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33263-1-AP (ANT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. Negative control: mouse brain tissue, mouse heart tissue.

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  IHC staining of mouse testis using 33263-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 33263-1-AP (SLC25A31/ANT4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 33263-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 33263-1-AP (SLC25A31/ANT4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 33263-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 33263-1-AP (SLC25A31/ANT4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 33263-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 33263-1-AP (SLC25A31/ANT4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U-251 using 33263-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using SLC25A31/ANT4 antibody (33263-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

SLC25A31/ANT4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 33263-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ANT4, SLC25A31, AAC4, Adenine nucleotide translocator 4, ADP,ATP carrier protein 4

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Tested Applications

• Positive WB detected in: human testis tissue, mouse testis tissue, rat testis tissue
• Positive IHC detected in: mouse kidney tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33263-1-AP targets SLC25A31/ANT4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11008

  Product name: Recombinant human SLC25A31 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-315 aa of BC022032

  Sequence: MHREPAKKKAEKRLFDASSFGKDLLAGGVAAAVSKTAVAPIERVKLLLQVQASSKQISPEARYKGMVDCLVRIPREQGFFSFWRGNLANVIRYFPTQALNFAFKDKYKQLFMSGVNKEKQFWRWFLANLASGGAAGATSLCVVYPLDFARTRLGVDIGKGPEERQFKGLGDCIMKIAKSDGIAGLYQGFGVSVQGIIVYRASYFGAYDTVKGLLPKPKKTPFLVSFFIAQVVTTCSGILSYPFDTVRRRMMMQSGEAKRQYKGTLDCFVKIYQHEGISSFFRGAFSNVLRGTGGALVLVLYDKIKEFFHIDIGGR

• Full Name: solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 31
• Calculated Molecular Weight: 315 aa, 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC022032
• Gene Symbol: SLC25A31
• Gene ID (NCBI): 83447
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9H0C2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC25A31, also known as ANT4, is a member of the mitochondrial carrier family. The proteins of this family are mainly involved in the transport of substances inside and outside the mitochondria. SLC25A31 is an ADP/ATP antiporter protein, which catalyzes the exchange of ATP generated in mitochondria by ATP synthase against ADP produced in cytosol by most energy-consuming reactions(PMID: 15670820). SLC25A31 is mainly expressed in the testis and male germ cells (PMID: 25031318, 23506884).