SLC25A32 Polyclonal antibody

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• KD/KO Validated

SLC25A32 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13080-1-AP

Synonyms

MFT, MFTC, SLC25A32

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Validation Data Gallery

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  WB analysis using 13080-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13080-1-AP (SLC25A32 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 13080-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13080-1-AP (SLC25A32 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 13080-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13080-1-AP (SLC25A32 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, MCF-7 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

13080-1-AP targets SLC25A32 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC25A32 fusion protein Ag3722
• Full Name: solute carrier family 25, member 32
• Calculated Molecular Weight: 315 aa, 35 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC021893
• Gene Symbol: SLC25A32
• Gene ID (NCBI): 81034
• RRID: AB_2877913
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Solute carrier family 25 member 32 (SLC25A32) can transfer tetrahydrofolate (THF) from cellular cytoplasm to the mitochondria as a mitochondrial folate transporter (MFT). It has been reported that SLC25A32 can also transport FAD across the mitochondrial inner membrane. Chronic alcohol exposure affects mitochondrial folate uptake and may affect the transcription of the SLC25A32. And functional SLC25A32 defect is associated with the cranial neural tube defects(PMID: 21956163; 29666258)

Publications

Species	Application	Title
human	WB,IHC	BMC Cancer; SLC25A32 promotes malignant progression of glioblastoma by activating PI3K-AKT signaling pathway; Authors - Zhiwei Xue ; KD Validated