SLC25A39 Polyclonal antibody

• Featured Product
• KD/KO Validated

SLC25A39 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

6

Reviews (1)

Cat no : 14963-1-AP

Synonyms

CGI 69, CGI69, PRO2163, SLC25A39

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Validation Data Gallery

• 

  WB analysis using 14963-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14963-1-AP (SLC25A39 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14963-1-AP

  mouse brain tissue was subjected to SDS PAGE followed by western blot with 14963-1-AP (SLC25A39 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 1 publications below

Product Information

14963-1-AP targets SLC25A39 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC25A39 fusion protein Ag6825
• Full Name: solute carrier family 25, member 39
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 37-39 kDa
• GenBank Accession Number: BC001398
• Gene Symbol: SLC25A39
• Gene ID (NCBI): 51629
• RRID: AB_2878095
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC25A39, a member of the mitochondrial carrier family, is a mitochondrial transporter for basic amino acids localized to mitochondria. SLC25A39 can be expressed in human testis, kidney, heart, brain, liver and spleen. It has been reported that SLC25A39 is essential for heme biosynthesis in mammals. SLC25A29 is associated with mitochondrial protein synthesis and amino acid degradation. (PMID: 19656490, 11139402, 24652292)

Publications

Species	Application	Title
human	WB,IF	Nature; SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.; Authors - Ying Wang ; KO Validated
human	WB	Nat Commun; Combinatorial GxGxE CRISPR screen identifies SLC25A39 in mitochondrial glutathione transport linking iron homeostasis to OXPHOS.; Authors - Xiaojian Shi
human	WB	Science; Autoregulatory control of mitochondrial glutathione homeostasis; Authors - Yuyang Liu
human	WB	Mol Cell; Dual regulation of SLC25A39 by AFG3L2 and iron controls mitochondrial glutathione homeostasis; Authors - Xiaojian Shi
mouse	WB	Redox Biol; S-Adenosyl-l-methionine restores brain mitochondrial membrane fluidity and GSH content improving Niemann-Pick type C disease; Authors - Leire Goicoechea
mouse	WB	Biol Trace Elem Res; Chronic Exposure to Arsenic and Fluoride Starting at Gestation Alters Liver Mitochondrial Protein Expression and Induces Early Onset of Liver Fibrosis in Male Mouse Offspring; Authors - Wendy L González-Alfonso

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ashish (Verified Customer) (12-18-2023)

n/a

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: EWING SARCOMA CELL LINE