SLC25A6-Specific Polyclonal antibody

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  WB analysis using 51031-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51031-1-AP (SLC25A6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 51031-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51031-1-AP (SLC25A6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 51031-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51031-1-AP (SLC25A6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 51031-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 51031-1-AP (SLC25A6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 51031-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 51031-1-AP (SLC25A6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLC25A6-Specific Polyclonal Antibody for WB, IHC, ELISA

Cat No. 51031-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

SLC25A6, AAC3, ADP,ATP carrier protein 3, ADP/ATP translocase 3, ADP/ATP translocase 3, N-terminally processed

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Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells, K-562 cells, NCI-H1299 cells
• Positive IHC detected in: human lung cancer tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

51031-1-AP targets SLC25A6-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 6
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 30-33 kDa
• GenBank Accession Number: BC031912
• Gene Symbol: SLC25A6
• Gene ID (NCBI): 293
• RRID: AB_2190370
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P12236
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC25A6, also known as ANT3 (adenine nucleotide translocase 3), is a member of the solute carrier family 25, specifically the mitochondrial carrier subfamily. As part of the mitochondrial carrier family, SLC25A6 plays a role in the mitochondrial energy production process by facilitating the exchange of metabolites necessary for oxidative phosphorylation (PMID: 23506884).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Int J Oncol; Identification of fatal outcome in a childhood nasopharyngeal carcinoma patient by protein expression profiling.; Authors - Mohamed E M Saeed
human	WB	MethodsX; Complete solubilization of mammalian cells in lysates; Authors - Izabela Bednarska