Tested Applications
| Positive WB detected in | Jurkat cells, HEK-293 cells, K-562 cells, NCI-H1299 cells |
| Positive IHC detected in | human lung cancer tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
51031-1-AP targets SLC25A6-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 6 |
| Calculated Molecular Weight | 33 kDa |
| Observed Molecular Weight | 30-33 kDa |
| GenBank Accession Number | BC031912 |
| Gene Symbol | SLC25A6 |
| Gene ID (NCBI) | 293 |
| RRID | AB_2190370 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P12236 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SLC25A6, also known as ANT3 (adenine nucleotide translocase 3), is a member of the solute carrier family 25, specifically the mitochondrial carrier subfamily. As part of the mitochondrial carrier family, SLC25A6 plays a role in the mitochondrial energy production process by facilitating the exchange of metabolites necessary for oxidative phosphorylation (PMID: 23506884).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SLC25A6-Specific antibody 51031-1-AP | Download protocol |
| WB protocol for SLC25A6-Specific antibody 51031-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SLC25A6-Specific Polyclonal antibody (Cat# 51031-1-AP) has 2 citations. They appear in Int J Oncol (2018), where it was used in protein expression profiling of childhood nasopharyngeal carcinoma, and MethodsX (2024), where it was applied for complete solubilization of mammalian cells in lysates via Western blot. The associated research fields are oncology (nasopharyngeal carcinoma) and cell biology/methods development.
| Species | Application | Title |
|---|---|---|
Int J Oncol Identification of fatal outcome in a childhood nasopharyngeal carcinoma patient by protein expression profiling. | ||
