SLC26A8 Polyclonal antibody

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  WB analysis using 12776-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12776-1-AP (SLC26A8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 12776-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12776-1-AP (SLC26A8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse testis using 12776-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12776-1-AP (SLC26A8 antibody), at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse testis using 12776-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12776-1-AP (SLC26A8 antibody), at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

SLC26A8 Polyclonal Antibody for WB, IF-P, ELISA

Cat No. 12776-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Synonyms

TAT1

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Tested Applications

• Positive WB detected in: human testis tissue, mouse testis tissue
• Positive IF-P detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

12776-1-AP targets SLC26A8 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3472

  Product name: Recombinant human SLC26A8 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 570-864 aa of BC025408

  Sequence: QQYEEVEEVWLPNNSSRNSSPGLPDVAESQGRRSLIPYSDASLLPSVHTIILDFSMVHYVDSRGLVVLRQICNAFQNANILILIAGCHSSIVRAFERNDFFDAGITKTQLFLSVHDAVLFALSRKVIGSSELSIDESETVIRETYSETDKNDNSRYKMSSSFLGSQKNVSPGFIKIQQPVEEESELDLELESEQEAGLGLDLDLDRELEPEMEPKAETETKTQTEMEPQPETEPEMEPNPKSRPRAHTFPQQRYWPMYHPSMASTQSQTQTRTWSVERRRHPMDSYSPEGNSNED

• Full Name: solute carrier family 26, member 8
• Calculated Molecular Weight: 970 aa, 109 kDa
• Observed Molecular Weight: 62, 86 and 97 kDa
• GenBank Accession Number: BC025408
• Gene Symbol: SLC26A8
• Gene ID (NCBI): 116369
• RRID: AB_2877880
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96RN1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC26A8, also named as TAT1, acts as a DIDS-sensitive anion exchanger mediating chloride, sulfate and oxalate transport. It has been reported that SLC26A8 is a sulfate transporter specifically expressed in spermatocytes and spermatids, playing a key role in spermatogenesis. There are several isoforms of SLC26A8 and 12776-1-AP antibody detects 62, 86 and 97 kDa protein in SDS-PAGE. (PMID: 11278976; 11834742)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Front Immunol; The role of intestinal immune cells and matrix metalloproteinases in inflammatory bowel disease; Authors - Kun Mei
human	IF	Asian J Androl; The role of SLC26A8 homozygous variants in male infertility and flagellum abnormalities; Authors - Bin Mao