SLC28A3 Polyclonal antibody

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  WB analysis using 18182-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18182-1-AP (SLC28A3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 18182-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 18182-1-AP (SLC28A3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 18182-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 18182-1-AP (SLC28A3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 18182-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using SLC28A3 antibody (18182-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

SLC28A3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 18182-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

hCNT3, Concentrative Na(+)-nucleoside cotransporter 3, CNT3, CNT 3

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Tested Applications

• Positive WB detected in: HL-60 cells, mouse kidney tissue, mouse liver tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18182-1-AP targets SLC28A3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12887

  Product name: Recombinant human SLC28A3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-186 aa of BC093821

  Sequence: MELRSTAAPRAEGYSNVGFQNEENFLENENTSGNNSIRSRAVQSREHTNTKQDEEQVTVEQDSPRNREHMEDDDEEMQQKGCLERRYDTVCGFCRKHKTTLRHIIWGILLAGYLVMVISACVLNFHRALPLFVITVAAIFFVVWDHLMAKYEHRIDEMLSPGRRLLNSHWFWLKWVIWSSLVLAVI

• Full Name: solute carrier family 28 (sodium-coupled nucleoside transporter), member 3
• Calculated Molecular Weight: 691 aa, 77 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC093821
• Gene Symbol: SLC28A3
• Gene ID (NCBI): 64078
• RRID: AB_3085558
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9HAS3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC28A3 (Solute carrier family 28 member 3), also named CNT3, has sodium-dependent, pyrimidine- and purine-selective (PMID: 11032837, PMID: 15861042, PMID: 16446384, PMID: 17140564, PMID: 21998139). It is involved in the homeostasis of endogenous nucleosides (PMID: 11032837, PMID: 15861042). SLC28A3 is able to transports uridine, gemcitabine, 3'-azido-3'-deoxythymidine (AZT), ribavirin and 3-deazauridine (PMID: 21795683, PMID: 11032837, PMID: 17140564). SLC28A3, the human concentrative nucleoside transporter, plays a role in mediating the cellular entry of a broad array of physiological nucleosides and synthetic anticancer nucleoside analog drugs (PMID: 15738947). Variants in SLC28A3 and UGT1A6 act as genetic markers predictive of anthracycline-induced cardiotoxicity (PMID: 23441093).