GLUT1 Recombinant antibody

GLUT1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

6F14

Cat no : 81463-1-RR

Synonyms

DYT17, DYT18, GLUT, GLUT 1, GLUT1, HepG2 glucose transporter, PED, SLC2A1



Tested Applications

Positive WB detected inunboiled HEK-293 cells, HeLa cells (incubated at 37°C)

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

81463-1-RR targets GLUT1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GLUT1 fusion protein Ag16282
Full Name solute carrier family 2 (facilitated glucose transporter), member 1
Calculated molecular weight 492 aa, 54 kDa
Observed molecular weight 45-55 kDa
GenBank accession numberBC121804
Gene symbol SLC2A1
Gene ID (NCBI) 6513
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GLUT1, also known as SLC2A1, is a ubiquitously expressed glucose transporter and is responsible for the basal level of glucose uptake in most cell types. Human erythrocytes express the highest level of GLUT1. Defects in SLC2A1 are the cause of GLUT1 deficiency syndrome type 1 and type 2. High expression of GLUT1 has been reported to be a reliable immunohistochemical marker for juvenile hemangiomas. GLUT1 protein may appear as two or more distinct forms among 43 kDa to 55 kDa due to the different glycosylation states. And the conversion of the highly glycosylated form of GLUT1 to a less glycosylated form has been reported to correlate with differentiation (PMID: 8263524, 23302780).

Protocols

Product Specific Protocols
WB protocol for GLUT1 antibody 81463-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols