SLC30A1 Polyclonal antibody

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  WB analysis using 22661-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22661-1-AP (SLC30A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

SLC30A1 Polyclonal Antibody for WB, ELISA

Cat No. 22661-1-AP

Publications(1)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Zinc transporter 1, ZNT1, ZnT-1

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Tested Applications

• Positive WB detected in: HeLa cells, HeLa membrane protein, HepG2 total crude protein, HepG2 membrane protein

It is best to use membrane samples for detection, for membrane proteins are hydrophobic and tend to aggregate when boiled at high temperatures.

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

22661-1-AP targets SLC30A1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18567

  Product name: Recombinant human SLC30A1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 330-507 aa of BC121015

  Sequence: YTTYPLLKESALILLQTVPKQIDIRNLIKELRNVEGVEEVHELHVWQLAGSRIIATAHIKCEDPTSYMEVAKTIKDVFHNHGIHATTIQPEFASVGSKSSVVPCELACRTQCALKQCCGTLPQAPSGKDAEKTPAVSISCLELSNNLEKKPRRTKAENIPAVVIEIKNMPNKQPESSL

• Full Name: solute carrier family 30 (zinc transporter), member 1
• Calculated Molecular Weight: 507 aa, 55 kDa
• Observed Molecular Weight: 63~75 kDa
• GenBank Accession Number: BC121015
• Gene Symbol: SLC30A1
• Gene ID (NCBI): 7779
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y6M5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zinc transporter 1 (ZNT1), also known as SLC30A1, is the only zinc transporter predominantly located on the plasma membrane, where it plays a pivotal role exporting cytosolic zinc to the extracellular space. SLC30A1 expression is posttranslationally regulated by cellular zinc status and can be N-glycosylated on Asn299 in the extracellular loop between transmembrane domains (PMID: 15451416, 18936201). ZnT1 is located in the plasma membrane and is responsible for Zn export from the cytosol to the extracellular space during periods of elevated cytosolic Zn. Western analysis detected a higher band at∼63 kDa than the expected molecular mass, which could be attributed to the properties of the cytosolic histidine-rich loop (PMID: 31471319, 15753530).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Eur J Histochem; HPV infection upregulates the expression of ZNT-1 in condyloma acuminatum.; Authors - Li Niu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

James (Verified Customer) (10-01-2025)

Excellent antibody. Have used at 1:2000 for Western blotting, detection by chemiluminescence.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Human In vitro Cancer Cells

FH

David (Verified Customer) (09-15-2025)

Concentration used 1:5000. works pretty good. Strong clean signal.

• Applications: Western Blot