Tested Applications
| Positive WB detected in | HeLa cells, HeLa membrane protein, HepG2 total crude protein, HepG2 membrane protein |
It is best to use membrane samples for detection, for membrane proteins are hydrophobic and tend to aggregate when boiled at high temperatures.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22661-1-AP targets SLC30A1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18567 Product name: Recombinant human SLC30A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 330-507 aa of BC121015 Sequence: YTTYPLLKESALILLQTVPKQIDIRNLIKELRNVEGVEEVHELHVWQLAGSRIIATAHIKCEDPTSYMEVAKTIKDVFHNHGIHATTIQPEFASVGSKSSVVPCELACRTQCALKQCCGTLPQAPSGKDAEKTPAVSISCLELSNNLEKKPRRTKAENIPAVVIEIKNMPNKQPESSL Predict reactive species |
| Full Name | solute carrier family 30 (zinc transporter), member 1 |
| Calculated Molecular Weight | 507 aa, 55 kDa |
| Observed Molecular Weight | 63~75 kDa |
| GenBank Accession Number | BC121015 |
| Gene Symbol | SLC30A1 |
| Gene ID (NCBI) | 7779 |
| RRID | AB_3742077 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6M5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Zinc transporter 1 (ZNT1), also known as SLC30A1, is the only zinc transporter predominantly located on the plasma membrane, where it plays a pivotal role exporting cytosolic zinc to the extracellular space. SLC30A1 expression is posttranslationally regulated by cellular zinc status and can be N-glycosylated on Asn299 in the extracellular loop between transmembrane domains (PMID: 15451416, 18936201). ZnT1 is located in the plasma membrane and is responsible for Zn export from the cytosol to the extracellular space during periods of elevated cytosolic Zn. Western analysis detected a higher band at∼63 kDa than the expected molecular mass, which could be attributed to the properties of the cytosolic histidine-rich loop (PMID: 31471319, 15753530).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC30A1 antibody 22661-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SLC30A1 (Zinc Transporter 1) polyclonal antibody, catalog number 22661-1-AP, has 4 total citations. These appear in Cell Reports Medicine, Biomedicines, the Journal of Biological Chemistry, and the European Journal of Histochemistry. Research fields span cancer immunotherapy (cuproptosis), immunomodulation and anti-inflammatory studies, copper metabolism and cytochrome c oxidase analysis, and HPV-related histopathology. Applications cited include Western blot and immunohistochemistry, with reactivity in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Rep Med Nilotinib induces immunogenic cuproptosis to potentiate cancer immunotherapy. | ||
Biomedicines Immunomodulatory and Anti-Inflammatory Effects of Phycocyanin Oligopeptide Combined with Selenium and Zinc in LPS-Induced Mice. | ||
J Biol Chem Dynamic assessment of the allocation of copper to cytochrome c oxidase using size-exclusion chromatography (SEC) combined with inductively coupled plasma mass spectrometry (ICP-MS).
| ||
Eur J Histochem HPV infection upregulates the expression of ZNT-1 in condyloma acuminatum. |
Reviews
What customers say
Both reviewers rated this antibody 5 stars for Western blotting. One user reported excellent performance at a 1:2000 dilution on human in vitro cancer cells with chemiluminescence detection. The other used a 1:5000 dilution and noted strong, clean signal. Overall, users find the antibody reliable and effective for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
James (Verified Customer) (10-01-2025) | Excellent antibody. Have used at 1:2000 for Western blotting, detection by chemiluminescence.
|
David (Verified Customer) (09-15-2025) | Concentration used 1:5000. works pretty good. Strong clean signal.
|
